Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy

Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy

Abstract The mucosal layer of conducting airways is the primary tissue exposed to inhaled microorganisms, allergens and pollutants. We developed an in vivo two-photon microscopic approach that allows performing dynamic imaging studies in the mouse trachea, which is a commonly used in vivo model of h...

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Main Authors: Tibor Z. Veres, Tamás Kopcsányi, Marko Tirri, Armin Braun, Masayuki Miyasaka, Ronald N. Germain, Sirpa Jalkanen, Marko Salmi
Format: Article
Language:English
Published: Nature Publishing Group 2017-04-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-00769-6
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spelling doaj-50d69311e3a24a059be6d618462735542020-12-08T00:55:27ZengNature Publishing GroupScientific Reports2045-23222017-04-01711910.1038/s41598-017-00769-6Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopyTibor Z. Veres0Tamás Kopcsányi1Marko Tirri2Armin Braun3Masayuki Miyasaka4Ronald N. Germain5Sirpa Jalkanen6Marko Salmi7MediCity Research Laboratory, University of TurkuMediCity Research Laboratory, University of TurkuInstitute of Biomedicine, Turku Center for Disease Modeling, and Turku PET Centre, University of TurkuDepartment of Preclinical Pharmacology and In Vitro Toxicology, Fraunhofer Institute for Toxicology and Experimental Medicine, and Institute of Immunology, MHH; Hannover, 30625 Germany; Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Centre for Lung Research (DZL)MediCity Research Laboratory, University of TurkuLymphocyte Biology Section, Laboratory of Systems Biology, National Institute of Allergy and Infectious Diseases, National Institutes of HealthMediCity Research Laboratory, University of TurkuMediCity Research Laboratory, University of TurkuAbstract The mucosal layer of conducting airways is the primary tissue exposed to inhaled microorganisms, allergens and pollutants. We developed an in vivo two-photon microscopic approach that allows performing dynamic imaging studies in the mouse trachea, which is a commonly used in vivo model of human small-diameter bronchi. By providing stabilized access to the tracheal mucosa without intubation, our setup uniquely allows dynamic in vivo imaging of mucociliary clearance and steady-state immune cell behavior within the complex airway mucosal tissue.https://doi.org/10.1038/s41598-017-00769-6
collection DOAJ
language English
format Article
sources DOAJ
author Tibor Z. Veres
Tamás Kopcsányi
Marko Tirri
Armin Braun
Masayuki Miyasaka
Ronald N. Germain
Sirpa Jalkanen
Marko Salmi
spellingShingle Tibor Z. Veres
Tamás Kopcsányi
Marko Tirri
Armin Braun
Masayuki Miyasaka
Ronald N. Germain
Sirpa Jalkanen
Marko Salmi
Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
Scientific Reports
author_facet Tibor Z. Veres
Tamás Kopcsányi
Marko Tirri
Armin Braun
Masayuki Miyasaka
Ronald N. Germain
Sirpa Jalkanen
Marko Salmi
author_sort Tibor Z. Veres
title Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
title_short Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
title_full Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
title_fullStr Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
title_full_unstemmed Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
title_sort intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2017-04-01
description Abstract The mucosal layer of conducting airways is the primary tissue exposed to inhaled microorganisms, allergens and pollutants. We developed an in vivo two-photon microscopic approach that allows performing dynamic imaging studies in the mouse trachea, which is a commonly used in vivo model of human small-diameter bronchi. By providing stabilized access to the tracheal mucosa without intubation, our setup uniquely allows dynamic in vivo imaging of mucociliary clearance and steady-state immune cell behavior within the complex airway mucosal tissue.
url https://doi.org/10.1038/s41598-017-00769-6
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AT tamaskopcsanyi intubationfreeinvivoimagingofthetrachealmucosausingtwophotonmicroscopy
AT markotirri intubationfreeinvivoimagingofthetrachealmucosausingtwophotonmicroscopy
AT arminbraun intubationfreeinvivoimagingofthetrachealmucosausingtwophotonmicroscopy
AT masayukimiyasaka intubationfreeinvivoimagingofthetrachealmucosausingtwophotonmicroscopy
AT ronaldngermain intubationfreeinvivoimagingofthetrachealmucosausingtwophotonmicroscopy
AT sirpajalkanen intubationfreeinvivoimagingofthetrachealmucosausingtwophotonmicroscopy
AT markosalmi intubationfreeinvivoimagingofthetrachealmucosausingtwophotonmicroscopy
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