Comparative Analysis of V-Akt Murine Thymoma Viral Oncogene Homolog 3 (AKT3) Gene between Cow and Buffalo Reveals Substantial Differences for Mastitis
AKT3 gene is a constituent of the serine/threonine protein kinase family and plays a crucial role in synthesis of milk fats and cholesterol by regulating activity of the sterol regulatory element binding protein (SREBP). AKT3 is highly conserved in mammals and its expression levels during the lactat...
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| Format: | Article |
| Language: | English |
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Hindawi Limited
2018-01-01
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| Series: | BioMed Research International |
| Online Access: | http://dx.doi.org/10.1155/2018/1463732 |
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doaj-50d61618381b416ab2f9e3e989008509 |
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Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Farman Ullah Dinesh Bhattarai Zhangrui Cheng Xianwei Liang Tingxian Deng Zia Ur Rehman Hira Sajjad Talpur Tesfaye Worku Rahim Dad Brohi Muhammad Safdar Muhammad Jamil Ahmad Mohammad Salim Momen Khan Hafiz Ishfaq Ahmad Shujun Zhang |
| spellingShingle |
Farman Ullah Dinesh Bhattarai Zhangrui Cheng Xianwei Liang Tingxian Deng Zia Ur Rehman Hira Sajjad Talpur Tesfaye Worku Rahim Dad Brohi Muhammad Safdar Muhammad Jamil Ahmad Mohammad Salim Momen Khan Hafiz Ishfaq Ahmad Shujun Zhang Comparative Analysis of V-Akt Murine Thymoma Viral Oncogene Homolog 3 (AKT3) Gene between Cow and Buffalo Reveals Substantial Differences for Mastitis BioMed Research International |
| author_facet |
Farman Ullah Dinesh Bhattarai Zhangrui Cheng Xianwei Liang Tingxian Deng Zia Ur Rehman Hira Sajjad Talpur Tesfaye Worku Rahim Dad Brohi Muhammad Safdar Muhammad Jamil Ahmad Mohammad Salim Momen Khan Hafiz Ishfaq Ahmad Shujun Zhang |
| author_sort |
Farman Ullah |
| title |
Comparative Analysis of V-Akt Murine Thymoma Viral Oncogene Homolog 3 (AKT3) Gene between Cow and Buffalo Reveals Substantial Differences for Mastitis |
| title_short |
Comparative Analysis of V-Akt Murine Thymoma Viral Oncogene Homolog 3 (AKT3) Gene between Cow and Buffalo Reveals Substantial Differences for Mastitis |
| title_full |
Comparative Analysis of V-Akt Murine Thymoma Viral Oncogene Homolog 3 (AKT3) Gene between Cow and Buffalo Reveals Substantial Differences for Mastitis |
| title_fullStr |
Comparative Analysis of V-Akt Murine Thymoma Viral Oncogene Homolog 3 (AKT3) Gene between Cow and Buffalo Reveals Substantial Differences for Mastitis |
| title_full_unstemmed |
Comparative Analysis of V-Akt Murine Thymoma Viral Oncogene Homolog 3 (AKT3) Gene between Cow and Buffalo Reveals Substantial Differences for Mastitis |
| title_sort |
comparative analysis of v-akt murine thymoma viral oncogene homolog 3 (akt3) gene between cow and buffalo reveals substantial differences for mastitis |
| publisher |
Hindawi Limited |
| series |
BioMed Research International |
| issn |
2314-6133 2314-6141 |
| publishDate |
2018-01-01 |
| description |
AKT3 gene is a constituent of the serine/threonine protein kinase family and plays a crucial role in synthesis of milk fats and cholesterol by regulating activity of the sterol regulatory element binding protein (SREBP). AKT3 is highly conserved in mammals and its expression levels during the lactation periods of cattle are markedly increased. AKT3 is highly expressed in the intestine followed by mammary gland and it is also expressed in immune cells. It is involved in the TLR pathways as effectively as proinflammatory cytokines. The aims of this study were to investigate the sequences differences between buffalo and cow. Our results showed that there were substantial differences between buffalo and cow in some exons and noteworthy differences of the gene size in different regions. We also identified the important consensus sequence motifs, variation in 2000 upstream of ATG, substantial difference in the “3′UTR” region, and miRNA association in the buffalo sequences compared with the cow. In addition, genetic analyses, such as gene structure, phylogenetic tree, position of different motifs, and functional domains, were performed to establish their correlation with other species. This may indicate that a buffalo breed has potential resistance to disease, environment changes, and airborne microorganisms and some good production and reproductive traits. |
| url |
http://dx.doi.org/10.1155/2018/1463732 |
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1724766678609297408 |
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doaj-50d61618381b416ab2f9e3e9890085092020-11-25T02:44:15ZengHindawi LimitedBioMed Research International2314-61332314-61412018-01-01201810.1155/2018/14637321463732Comparative Analysis of V-Akt Murine Thymoma Viral Oncogene Homolog 3 (AKT3) Gene between Cow and Buffalo Reveals Substantial Differences for MastitisFarman Ullah0Dinesh Bhattarai1Zhangrui Cheng2Xianwei Liang3Tingxian Deng4Zia Ur Rehman5Hira Sajjad Talpur6Tesfaye Worku7Rahim Dad Brohi8Muhammad Safdar9Muhammad Jamil Ahmad10Mohammad Salim11Momen Khan12Hafiz Ishfaq Ahmad13Shujun Zhang14Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Pathobiology and Population Sciences, Royal Veterinary College, Hawkshead Lane, Hatfield, Hertfordshire AL9 7TA, UKThe Opening Project of Guangxi Key Laboratory of Buffalo Genetics, Reproduction and Breeding, Nanning, Guangxi 530000, ChinaThe Opening Project of Guangxi Key Laboratory of Buffalo Genetics, Reproduction and Breeding, Nanning, Guangxi 530000, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Forestry and Wildlife Management, The University of Haripur, Khyber Pakhtunkhwa, PakistanLivestock and Dairy Development, Khyber Pakhtunkhwa, PakistanKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaAKT3 gene is a constituent of the serine/threonine protein kinase family and plays a crucial role in synthesis of milk fats and cholesterol by regulating activity of the sterol regulatory element binding protein (SREBP). AKT3 is highly conserved in mammals and its expression levels during the lactation periods of cattle are markedly increased. AKT3 is highly expressed in the intestine followed by mammary gland and it is also expressed in immune cells. It is involved in the TLR pathways as effectively as proinflammatory cytokines. The aims of this study were to investigate the sequences differences between buffalo and cow. Our results showed that there were substantial differences between buffalo and cow in some exons and noteworthy differences of the gene size in different regions. We also identified the important consensus sequence motifs, variation in 2000 upstream of ATG, substantial difference in the “3′UTR” region, and miRNA association in the buffalo sequences compared with the cow. In addition, genetic analyses, such as gene structure, phylogenetic tree, position of different motifs, and functional domains, were performed to establish their correlation with other species. This may indicate that a buffalo breed has potential resistance to disease, environment changes, and airborne microorganisms and some good production and reproductive traits.http://dx.doi.org/10.1155/2018/1463732 |