Characterisation of Constitutive Promoters from the Anderson library in Chromobacterium violaceum ATCC 12472

• Main Authors: Lu Ting Liow, Maybelle Darlene Kho Go, Wen Shan Yew
• Format: Article
• Language: English
• Published: Wiley 2019-06-01
• Series: Engineering Biology
• Subjects: biotechnology; molecular biophysics; biological organs; microorganisms; genetics; cellular biophysics; genomics; c; c. violaceum; synthetic biology genetic constructs; anderson library constitutive promoters; original characterized strengths; promoter strengths; automated microplate reader; 100-fold range; characterised genetic parts; diverse metabolic energetics; cyanogenic properties; potential industrially relevant chassis; chromobacterium violaceum atcc
• Online Access: https://digital-library.theiet.org/content/journals/10.1049/enb.2018.5007

Chromobacterium violaceum is a potential industrially relevant chassis due to its cyanogenic properties and diverse metabolic energetics. However, its application in synthetic biology is limited by the lack of characterised genetic parts. Six constitutive promoters of a 100-fold range in gene expression from the Anderson library were characterised in C. violaceum using an automated microplate reader. The datasheet provides an insight to the highly similar trend in promoter strengths in C. violaceum to the original characterised strengths of the Anderson library constitutive promoters in E. coli. This provides a foundation for constitutive promoters of different strengths as parts to be used for synthetic biology genetic constructs in C. violaceum.