Canine amniotic membrane derived mesenchymal stem cells exosomes addition in canine sperm freezing medium
Amniotic membrane stem cells are considered as a good alternative to embryonic stem cells, but their use in clinical studies is still not common. Here, exosomes from canine amniotic membrane mesenchymal stem cells (cAmMSCexo) were used for dog sperm cryopreservation. Upon cryopreserved straws using...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
The Korean Society of Animal Reproduction and Biotechnology
2020-09-01
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| Series: | Journal of Animal Reproduction and Biotechnology |
| Subjects: | |
| Online Access: | http://www.e-jarb.org/journal/view.html?uid=2547&vmd=Full |
| Summary: | Amniotic membrane stem cells are considered as a good alternative to embryonic stem cells, but their use in clinical studies is still not common. Here, exosomes from canine amniotic membrane mesenchymal stem cells (cAmMSCexo) were used for dog sperm cryopreservation. Upon cryopreserved straws using cryoprotectant containing 0, 0.5, 1, or 2 μg/mL of cAmMSC-exo were thawed, motility and membrane integrity were analyzed. However, results showed no significant differences between the groups. We concluded that cAmMSC-exo with lower than 2 µg/mL have no effects on sperm cryopreservation, and further studies to get higher concentrations of cAmMSC-exo should be conducted for clinical application. |
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| ISSN: | 2671-4639 2671-4663 |