Upper respiratory tract bacteria in Influenza-like illness cases in Indonesia using multiplex PCR method

• Main Authors: Vivi Setiawaty, Kartika Dewi Puspa, Kindi Adam, Krisna NA Pangesti
• Format: Article
• Language: English
• Published: Badan Penelitian dan Pengembangan Kesehatan 2014-02-01
• Series: Health Science Journal of Indonesia
• Subjects: bacterial infection, ILI, multiplex PCR
• Online Access: http://ejournal.litbang.kemkes.go.id/index.php/HSJI/article/view/3437
• ISSN: 2087-7021; 2338-3437

<p>Abstrak<br />Latar belakang: Walaupun surveilans ILI di Indonesia sudah berlangsung sejak tahun 2006, namun belum diketahui data mengenai bakteri-bakteri yang dapat dideteksi dari kasus ILI dan kemungkinan menyebabkan ILI.<br />Metode: Penelitian dilakukan pada bulan Maret – Desember tahun 2012, laboratorium Virologi Pusat Biomedis dan Teknologi Dasar Kesehatan menerima spesimen apus tenggorokan dan apus hidung dari 9 puskesmas yang menjadi sentinel surveilan ILI. Spesimen diperiksa menggunakan multipleks PCR untuk mendeteksi 6 panel bakteri.<br />Hasil:Sebanyak 175 spesimen kasus ILI bakteri yang paling banyak ditemukan pada spesimen ini adalah Streptococcus pneumoniae (n=163), Haemophilus influenzae (n=146) dan Legionella pneumophila (41).<br />Kesimpulan:Pada pasien ILI bakteri yang biasa terdeteksi pada saluran pernapasan bagian atas adalah Streptococcus  pneumoniae, Haemophilus  influenzae  dan  Legionella  pneumophila.  (Health Science <br />Indones 2013;2:83-6)</p><p>Kata kunci:infeksi bakteri, ILI, PCR multipleks</p><p>Abstract<br />Background: ILI surveillance in Indonesia has been conducted since 2006, but no data on the bacteria can be detected and caused ILI has been obtained. Method: From March to December 2012, Center for Research on Biomedic and Basic Health Technology’s laboratory was receiving throat and nose swab specimens from nine Public Health Centers appointed as <br />the sentinels for ILI surveillance. These specimens were analyzed using multiplex PCR method, which detected six panels of bacteria. Results: 175 specimens taken from ILI patients contained three most frequently found species of bacteria: Streptococcus pneumoniae (n=163), Haemophilus influenzae (n=146) andLegionella pneumophila (41). Conclusion: In ILI patients, there are several bacteria most frequently detected in upper respiratory tract such as Streptococcus pneumoniae, Haemophilus influenzae and Legionella pneumophila. (Health Science Indonesia 2013;2:83-6)</p><p>Key words:bacterial infection, ILI, multiplex PCR</p>