The production of IL-12β by mononuclear leucocytes of peripheral blood depending on clinical forms of pulmonary tuberculosis

The aim of this study was to evaluate the level of secretion of IL-12β by mononuclear leukocytes of peripheral blood under infiltrative and disseminated pulmonary tuberculosis. Blood mononuclear cells were isolated by gradient centrifugation, then cultured in complete culture medium; the level of IL...

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Main Authors:	R. R. Khasanova, O. I. Urazova, Z. K. Khaitova, O. V. Voronkova, V. V. Novitsky
Format:	Article
Language:	English
Published:	Siberian State Medical University (Tomsk) 2012-12-01
Series:	Bûlleten' Sibirskoj Mediciny
Subjects:	pulmonary tuberculosis antigen-presenting cells il-12β
Online Access:	https://bulletin.tomsk.ru/jour/article/view/915

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spelling	doaj-50aeb8c112984b9ba66f0fe51b5e45432021-07-29T08:37:58ZengSiberian State Medical University (Tomsk)Bûlleten' Sibirskoj Mediciny1682-03631819-36842012-12-0111621822010.20538/1682-0363-2012-6-218-220698The production of IL-12β by mononuclear leucocytes of peripheral blood depending on clinical forms of pulmonary tuberculosisR. R. Khasanova0O. I. Urazova1Z. K. Khaitova2O. V. Voronkova3V. V. Novitsky4Сибирский государственный медицинский университет, г. ТомскСибирский государственный медицинский университет, г. ТомскСибирский государственный медицинский университет, г. ТомскСибирский государственный медицинский университет, г. ТомскСибирский государственный медицинский университет, г. ТомскThe aim of this study was to evaluate the level of secretion of IL-12β by mononuclear leukocytes of peripheral blood under infiltrative and disseminated pulmonary tuberculosis. Blood mononuclear cells were isolated by gradient centrifugation, then cultured in complete culture medium; the level of IL-12β production was determined by the ELISA (ELISA). Peripheral blood served as the material of the research. It has been established, that under infiltrative pulmonary tuberculosis the levels of basal, protein and lipid-induced IL-12β production were significantly lower than in healthy donors and patients with disseminated pulmonary tuberculosis. Under disseminated pulmonary tuberculosis basal IL-12β production varies within normal limits; when stimulated by protein antigen, it increases, when influenced by lipid antigen — decreases.https://bulletin.tomsk.ru/jour/article/view/915pulmonary tuberculosisantigen-presenting cellsil-12β
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	R. R. Khasanova O. I. Urazova Z. K. Khaitova O. V. Voronkova V. V. Novitsky
spellingShingle	R. R. Khasanova O. I. Urazova Z. K. Khaitova O. V. Voronkova V. V. Novitsky The production of IL-12β by mononuclear leucocytes of peripheral blood depending on clinical forms of pulmonary tuberculosis Bûlleten' Sibirskoj Mediciny pulmonary tuberculosis antigen-presenting cells il-12β
author_facet	R. R. Khasanova O. I. Urazova Z. K. Khaitova O. V. Voronkova V. V. Novitsky
author_sort	R. R. Khasanova
title	The production of IL-12β by mononuclear leucocytes of peripheral blood depending on clinical forms of pulmonary tuberculosis
title_short	The production of IL-12β by mononuclear leucocytes of peripheral blood depending on clinical forms of pulmonary tuberculosis
title_full	The production of IL-12β by mononuclear leucocytes of peripheral blood depending on clinical forms of pulmonary tuberculosis
title_fullStr	The production of IL-12β by mononuclear leucocytes of peripheral blood depending on clinical forms of pulmonary tuberculosis
title_full_unstemmed	The production of IL-12β by mononuclear leucocytes of peripheral blood depending on clinical forms of pulmonary tuberculosis
title_sort	production of il-12β by mononuclear leucocytes of peripheral blood depending on clinical forms of pulmonary tuberculosis
publisher	Siberian State Medical University (Tomsk)
series	Bûlleten' Sibirskoj Mediciny
issn	1682-0363 1819-3684
publishDate	2012-12-01
description	The aim of this study was to evaluate the level of secretion of IL-12β by mononuclear leukocytes of peripheral blood under infiltrative and disseminated pulmonary tuberculosis. Blood mononuclear cells were isolated by gradient centrifugation, then cultured in complete culture medium; the level of IL-12β production was determined by the ELISA (ELISA). Peripheral blood served as the material of the research. It has been established, that under infiltrative pulmonary tuberculosis the levels of basal, protein and lipid-induced IL-12β production were significantly lower than in healthy donors and patients with disseminated pulmonary tuberculosis. Under disseminated pulmonary tuberculosis basal IL-12β production varies within normal limits; when stimulated by protein antigen, it increases, when influenced by lipid antigen — decreases.
topic	pulmonary tuberculosis antigen-presenting cells il-12β
url	https://bulletin.tomsk.ru/jour/article/view/915
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The production of IL-12β by mononuclear leucocytes of peripheral blood depending on clinical forms of pulmonary tuberculosis

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