Identification of a differentially expressed gene, <it>ACL</it>, between Meishan × Large White and Large White × Meishan F1 hybrids and their parents

• Main Authors: Xiong Yuan-Zhua, Jiang Si-Wen, Deng Chang-Yan, Zheng Rong, Xu De-Quan, Li Feng-E, Zuo Bo, Lei Ming-Gang, Wang Lin-Jie, Xu Yong-Jie, Wang Yan, Ren Zhu-Qing
• Format: Article
• Language: deu
• Published: BMC 2008-11-01
• Series: Genetics Selection Evolution
• Subjects: differential gene expression; ATP-citrate lyase; promoter; mutation; pigs
• Online Access: http://www.gsejournal.org/content/40/6/625

<p>Abstract</p> <p>ATP-citrate lyase (ACL), one of the lipogenic enzymes, catalyses the formation of acetyl-coenzyme A (CoA) involved in the synthesis of fatty acid and cholesterol. In pig, very little is known about the <it>ACL </it>gene. In this work, the mRNA differential display technique was used to analyse the differences in gene expression between Meishan and Large White pigs and the F1 hybrids of both direct and reciprocal crosses. Our results show that among the differentially expressed genes <it>ACL </it>is up-regulated in the backfat of the F1 hybrids. After cloning and analysing the fulllength cDNA and the 870 bp 5'-flanking sequence of the porcine <it>ACL </it>gene, a <it>C/T </it>mutation at position -97 bp upstream of the transcription site was detected. Luciferase activity detection showed that this mutation changed the transcriptional activity. In F1 hybrids, the heterozygous genotype <it>CT </it>was more frequent than the homozygous genotypes <it>CC </it>and <it>TT</it>. Real-time PCR analysis showed that in Meishan pigs, <it>ACL </it>mRNA expression was more abundant in individuals with genotype <it>CT </it>than in those with genotype <it>CC </it>or <it>TT </it>or in Large White pigs. These results indicate that the <it>C/T </it>mutation affects <it>ACL </it>mRNA expression, probably <it>via </it>the activator protein 2.</p>