Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers
Camellia huana is an endangered species with a narrow distribution in limestone hills of northern Guangxi and southern Guizhou provinces, China. We used one chloroplast DNA (cpDNA) fragment and 12 pairs of microsatellite (simple sequence repeat; SSR) markers to assess the genetic diversity and struc...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
KeAi Communications Co., Ltd.
2020-10-01
|
Series: | Plant Diversity |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2468265920300433 |
id |
doaj-5090116e2f7c4f5a885b2e19d597692a |
---|---|
record_format |
Article |
spelling |
doaj-5090116e2f7c4f5a885b2e19d597692a2021-04-02T13:02:29ZengKeAi Communications Co., Ltd.Plant Diversity2468-26592020-10-01425343350Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markersShuang Li0Shang-Li Liu1Si-Yu Pei2Man-Man Ning3Shao-Qing Tang4Guangxi Key Laboratory of Rare and Endangered Animal Ecology, College of Life Science, Guangxi Normal University, Guilin, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin, ChinaGuangxi Key Laboratory of Rare and Endangered Animal Ecology, College of Life Science, Guangxi Normal University, Guilin, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin, ChinaGuangxi Key Laboratory of Rare and Endangered Animal Ecology, College of Life Science, Guangxi Normal University, Guilin, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin, ChinaLongtan Nature Reserve Management Center, Hechi, ChinaGuangxi Key Laboratory of Rare and Endangered Animal Ecology, College of Life Science, Guangxi Normal University, Guilin, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin, China; Corresponding author. College of Life Science, Guangxi Normal University, Guilin 541006, China.Camellia huana is an endangered species with a narrow distribution in limestone hills of northern Guangxi and southern Guizhou provinces, China. We used one chloroplast DNA (cpDNA) fragment and 12 pairs of microsatellite (simple sequence repeat; SSR) markers to assess the genetic diversity and structure of 12 C. huana populations. A total of 99 alleles were detected for 12 polymorphic loci, and eight haplotypes and nine polymorphic sites were detected within 5200 bp of cpDNA. C. huana populations showed a low level of genetic diversity (n = 8, Hd = 0.759, Pi = 0.00042 for cpDNA, NA = 3.931, HE = 0.466 for SSRs), but high genetic differentiation between populations (FST = 0.2159 for SSRs, FST = 0.9318 for cpDNA). This can be attributed to the narrow distribution and limestone habitat of C. huana. STRUCTURE analysis divided natural C. huana populations into two groups, consistent with their geographical distribution. Thus, we suggest that five natural C. huana populations should be split into two units to be managed effectively.http://www.sciencedirect.com/science/article/pii/S2468265920300433Camellia huanaGenetic diversityGenetic structureConservation implications |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shuang Li Shang-Li Liu Si-Yu Pei Man-Man Ning Shao-Qing Tang |
spellingShingle |
Shuang Li Shang-Li Liu Si-Yu Pei Man-Man Ning Shao-Qing Tang Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers Plant Diversity Camellia huana Genetic diversity Genetic structure Conservation implications |
author_facet |
Shuang Li Shang-Li Liu Si-Yu Pei Man-Man Ning Shao-Qing Tang |
author_sort |
Shuang Li |
title |
Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers |
title_short |
Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers |
title_full |
Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers |
title_fullStr |
Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers |
title_full_unstemmed |
Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers |
title_sort |
genetic diversity and population structure of camellia huana (theaceae), a limestone species with narrow geographic range, based on chloroplast dna sequence and microsatellite markers |
publisher |
KeAi Communications Co., Ltd. |
series |
Plant Diversity |
issn |
2468-2659 |
publishDate |
2020-10-01 |
description |
Camellia huana is an endangered species with a narrow distribution in limestone hills of northern Guangxi and southern Guizhou provinces, China. We used one chloroplast DNA (cpDNA) fragment and 12 pairs of microsatellite (simple sequence repeat; SSR) markers to assess the genetic diversity and structure of 12 C. huana populations. A total of 99 alleles were detected for 12 polymorphic loci, and eight haplotypes and nine polymorphic sites were detected within 5200 bp of cpDNA. C. huana populations showed a low level of genetic diversity (n = 8, Hd = 0.759, Pi = 0.00042 for cpDNA, NA = 3.931, HE = 0.466 for SSRs), but high genetic differentiation between populations (FST = 0.2159 for SSRs, FST = 0.9318 for cpDNA). This can be attributed to the narrow distribution and limestone habitat of C. huana. STRUCTURE analysis divided natural C. huana populations into two groups, consistent with their geographical distribution. Thus, we suggest that five natural C. huana populations should be split into two units to be managed effectively. |
topic |
Camellia huana Genetic diversity Genetic structure Conservation implications |
url |
http://www.sciencedirect.com/science/article/pii/S2468265920300433 |
work_keys_str_mv |
AT shuangli geneticdiversityandpopulationstructureofcamelliahuanatheaceaealimestonespecieswithnarrowgeographicrangebasedonchloroplastdnasequenceandmicrosatellitemarkers AT shangliliu geneticdiversityandpopulationstructureofcamelliahuanatheaceaealimestonespecieswithnarrowgeographicrangebasedonchloroplastdnasequenceandmicrosatellitemarkers AT siyupei geneticdiversityandpopulationstructureofcamelliahuanatheaceaealimestonespecieswithnarrowgeographicrangebasedonchloroplastdnasequenceandmicrosatellitemarkers AT manmanning geneticdiversityandpopulationstructureofcamelliahuanatheaceaealimestonespecieswithnarrowgeographicrangebasedonchloroplastdnasequenceandmicrosatellitemarkers AT shaoqingtang geneticdiversityandpopulationstructureofcamelliahuanatheaceaealimestonespecieswithnarrowgeographicrangebasedonchloroplastdnasequenceandmicrosatellitemarkers |
_version_ |
1721566712329928704 |