Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers

Camellia huana is an endangered species with a narrow distribution in limestone hills of northern Guangxi and southern Guizhou provinces, China. We used one chloroplast DNA (cpDNA) fragment and 12 pairs of microsatellite (simple sequence repeat; SSR) markers to assess the genetic diversity and struc...

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Main Authors: Shuang Li, Shang-Li Liu, Si-Yu Pei, Man-Man Ning, Shao-Qing Tang
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2020-10-01
Series:Plant Diversity
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2468265920300433
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spelling doaj-5090116e2f7c4f5a885b2e19d597692a2021-04-02T13:02:29ZengKeAi Communications Co., Ltd.Plant Diversity2468-26592020-10-01425343350Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markersShuang Li0Shang-Li Liu1Si-Yu Pei2Man-Man Ning3Shao-Qing Tang4Guangxi Key Laboratory of Rare and Endangered Animal Ecology, College of Life Science, Guangxi Normal University, Guilin, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin, ChinaGuangxi Key Laboratory of Rare and Endangered Animal Ecology, College of Life Science, Guangxi Normal University, Guilin, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin, ChinaGuangxi Key Laboratory of Rare and Endangered Animal Ecology, College of Life Science, Guangxi Normal University, Guilin, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin, ChinaLongtan Nature Reserve Management Center, Hechi, ChinaGuangxi Key Laboratory of Rare and Endangered Animal Ecology, College of Life Science, Guangxi Normal University, Guilin, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin, China; Corresponding author. College of Life Science, Guangxi Normal University, Guilin 541006, China.Camellia huana is an endangered species with a narrow distribution in limestone hills of northern Guangxi and southern Guizhou provinces, China. We used one chloroplast DNA (cpDNA) fragment and 12 pairs of microsatellite (simple sequence repeat; SSR) markers to assess the genetic diversity and structure of 12 C. huana populations. A total of 99 alleles were detected for 12 polymorphic loci, and eight haplotypes and nine polymorphic sites were detected within 5200 bp of cpDNA. C. huana populations showed a low level of genetic diversity (n = 8, Hd = 0.759, Pi = 0.00042 for cpDNA, NA = 3.931, HE = 0.466 for SSRs), but high genetic differentiation between populations (FST = 0.2159 for SSRs, FST = 0.9318 for cpDNA). This can be attributed to the narrow distribution and limestone habitat of C. huana. STRUCTURE analysis divided natural C. huana populations into two groups, consistent with their geographical distribution. Thus, we suggest that five natural C. huana populations should be split into two units to be managed effectively.http://www.sciencedirect.com/science/article/pii/S2468265920300433Camellia huanaGenetic diversityGenetic structureConservation implications
collection DOAJ
language English
format Article
sources DOAJ
author Shuang Li
Shang-Li Liu
Si-Yu Pei
Man-Man Ning
Shao-Qing Tang
spellingShingle Shuang Li
Shang-Li Liu
Si-Yu Pei
Man-Man Ning
Shao-Qing Tang
Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers
Plant Diversity
Camellia huana
Genetic diversity
Genetic structure
Conservation implications
author_facet Shuang Li
Shang-Li Liu
Si-Yu Pei
Man-Man Ning
Shao-Qing Tang
author_sort Shuang Li
title Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers
title_short Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers
title_full Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers
title_fullStr Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers
title_full_unstemmed Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers
title_sort genetic diversity and population structure of camellia huana (theaceae), a limestone species with narrow geographic range, based on chloroplast dna sequence and microsatellite markers
publisher KeAi Communications Co., Ltd.
series Plant Diversity
issn 2468-2659
publishDate 2020-10-01
description Camellia huana is an endangered species with a narrow distribution in limestone hills of northern Guangxi and southern Guizhou provinces, China. We used one chloroplast DNA (cpDNA) fragment and 12 pairs of microsatellite (simple sequence repeat; SSR) markers to assess the genetic diversity and structure of 12 C. huana populations. A total of 99 alleles were detected for 12 polymorphic loci, and eight haplotypes and nine polymorphic sites were detected within 5200 bp of cpDNA. C. huana populations showed a low level of genetic diversity (n = 8, Hd = 0.759, Pi = 0.00042 for cpDNA, NA = 3.931, HE = 0.466 for SSRs), but high genetic differentiation between populations (FST = 0.2159 for SSRs, FST = 0.9318 for cpDNA). This can be attributed to the narrow distribution and limestone habitat of C. huana. STRUCTURE analysis divided natural C. huana populations into two groups, consistent with their geographical distribution. Thus, we suggest that five natural C. huana populations should be split into two units to be managed effectively.
topic Camellia huana
Genetic diversity
Genetic structure
Conservation implications
url http://www.sciencedirect.com/science/article/pii/S2468265920300433
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