Karyotype analysis of some Iranian native dog population
Today, Karyotying studies are important to understand the genetic structure of animals. In the current research to evaluate the chromosomal structure of some Iranian native dogs, blood samples were collected from the Kurdi, Sarabi and samples of Alborz province. Blood samples were cultured in define...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | fas |
Published: |
Shahid Bahonar University of Kerman
2015-11-01
|
Series: | مجله بیوتکنولوژی کشاورزی |
Subjects: | |
Online Access: | https://jab.uk.ac.ir/article_1140_a7a4e4c607a75a0d23d45205b6d86144.pdf |
Summary: | Today, Karyotying studies are important to understand the genetic structure of animals. In the current research to evaluate the chromosomal structure of some Iranian native dogs, blood samples were collected from the Kurdi, Sarabi and samples of Alborz province. Blood samples were cultured in defined medium for 72 h at 37 ˚С. Then the cell divisions were stopped at metaphase stage and chromosomal specimens were prepared for karyotype analysis. Next, centromeric index, total length of chromosome, chromosomal arm ratios and relative length of chromosomes were analyzed and the ideograms were created. The results indicated that the dog genomes contain 78 chromosomes including 38 acrocentric pairs in each sex, a pair of metacentric X in males and females and one metacentric Y chromosome in males. The average relative length of the autosomal chromosomes ranges from 1.13 to 4.89 μm in males and 1.10 to 5.21 μm in females. The ANOVA of the chromosomal data indicated that significant (P≤0.05) differences in chromosomal parameters were observed in animals. The results indicate a bias of chromosome asymmetry in animals which could be related to evolutions of the canine chromosomal structures. |
---|---|
ISSN: | 2228-6705 2228-6500 |