Development of a single cell spherical shell model for an investigation of electrical properties with a computing program

• Main Authors: Boonlamp, M., Bunthawin, S.
• Format: Article
• Language: English
• Published: Prince of Songkla University 2005-03-01
• Series: Songklanakarin Journal of Science and Technology (SJST)
• Subjects: dielectric dispersion; double-shell model; dielectrophoresis; computer program; dielectric constant; conductivity
• Online Access: http://www.sjst.psu.ac.th/journal/27-2-pdf/17cell.pdf

A spherical double shell model (SDM) for a single cell has been developed, using Laplace’s equation in spherical coordinates and boundary conditions. Electric field intensities and dielectric constants of each region inside and outside of the cell have been estimated. The dielectrophoretic spectrum of the real part of a complex function (Re[f ( ω)] were computed using Visual Foxpro Version 6, which gave calculated values pertaining to electrical properties of the cell model as compared with experimental values. The process was repeated until the error percentile was in an acceptable range. The calculated parameters were the dielectric constants and the conductivities of the inner cytoplasm ( εic, σic), the outer cytoplasm ( εoc, σoc), the inner membrane ( εim, σim), the outer membrane ( εom, σom), the suspending solution( εs, σs) and the thickness of each layer (dom, doc, dim), respectively. This computer program provides estimated values of cell electrical properties with high accuracy and required minimal computational time.