Isolation of S-Layer from Caulobacter and re-crystallization on planar supports

 Introduction: Self- assemble structures play an important role in many aspects of nanotechnology field such as biosesnsors, drug delivery, tissue engineering and targeting activity. S-layers proteins are an example of such molecules. These molecules possess the ability of re-cryst...

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Main Authors: Neda Habibi, tayebe zohrabi
Format: Article
Language:English
Published: University of Isfahan 2015-06-01
Series:Biological Journal of Microorganism
Subjects:
Online Access:http://uijs.ui.ac.ir/bjm/browse.php?a_code=A-10-152-1&slc_lang=en&sid=1
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spelling doaj-503158c1bf944a22bce35c8dcdb19ea62020-11-25T00:32:54ZengUniversity of IsfahanBiological Journal of Microorganism2322-51732322-51812015-06-014135766Isolation of S-Layer from Caulobacter and re-crystallization on planar supportsNeda Habibi0tayebe zohrabi1 Isfahan University of technology M. Sc. Student of Nanobiotechnology, University of Isfahan, Iran  Introduction: Self- assemble structures play an important role in many aspects of nanotechnology field such as biosesnsors, drug delivery, tissue engineering and targeting activity. S-layers proteins are an example of such molecules. These molecules possess the ability of re-crystallization after isolation from the surface of bacteria and therefore could be used for fabrication of assembled functional groups. Materials and methods: To this regard in this work S- Layers were isolated from Caulobacter and were assembled on mica and silicon wafers. S-Layers re-crystallization were characterized by means of AFM. In order to evaluate the production of assembled functional groups Immunological methods were used. Results: S-Layer with molecular weight of 96 KDa were successfully isolated from the surface of Caulobacter and crystallized on solid silicone and mica support. AFM results indicated assembled domains on mica and silicone. IgG immobilization was induced in the presence of S-Layer structures. Discussion and conclusion: The results confirmed the effect of S-Layer crystallization on fabrication of assembled functional groups.http://uijs.ui.ac.ir/bjm/browse.php?a_code=A-10-152-1&slc_lang=en&sid=1Self- assembly S- Layer Functional group
collection DOAJ
language English
format Article
sources DOAJ
author Neda Habibi
tayebe zohrabi
spellingShingle Neda Habibi
tayebe zohrabi
Isolation of S-Layer from Caulobacter and re-crystallization on planar supports
Biological Journal of Microorganism
Self- assembly
S- Layer
Functional group
author_facet Neda Habibi
tayebe zohrabi
author_sort Neda Habibi
title Isolation of S-Layer from Caulobacter and re-crystallization on planar supports
title_short Isolation of S-Layer from Caulobacter and re-crystallization on planar supports
title_full Isolation of S-Layer from Caulobacter and re-crystallization on planar supports
title_fullStr Isolation of S-Layer from Caulobacter and re-crystallization on planar supports
title_full_unstemmed Isolation of S-Layer from Caulobacter and re-crystallization on planar supports
title_sort isolation of s-layer from caulobacter and re-crystallization on planar supports
publisher University of Isfahan
series Biological Journal of Microorganism
issn 2322-5173
2322-5181
publishDate 2015-06-01
description  Introduction: Self- assemble structures play an important role in many aspects of nanotechnology field such as biosesnsors, drug delivery, tissue engineering and targeting activity. S-layers proteins are an example of such molecules. These molecules possess the ability of re-crystallization after isolation from the surface of bacteria and therefore could be used for fabrication of assembled functional groups. Materials and methods: To this regard in this work S- Layers were isolated from Caulobacter and were assembled on mica and silicon wafers. S-Layers re-crystallization were characterized by means of AFM. In order to evaluate the production of assembled functional groups Immunological methods were used. Results: S-Layer with molecular weight of 96 KDa were successfully isolated from the surface of Caulobacter and crystallized on solid silicone and mica support. AFM results indicated assembled domains on mica and silicone. IgG immobilization was induced in the presence of S-Layer structures. Discussion and conclusion: The results confirmed the effect of S-Layer crystallization on fabrication of assembled functional groups.
topic Self- assembly
S- Layer
Functional group
url http://uijs.ui.ac.ir/bjm/browse.php?a_code=A-10-152-1&slc_lang=en&sid=1
work_keys_str_mv AT nedahabibi isolationofslayerfromcaulobacterandrecrystallizationonplanarsupports
AT tayebezohrabi isolationofslayerfromcaulobacterandrecrystallizationonplanarsupports
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