Isolation of S-Layer from Caulobacter and re-crystallization on planar supports
Introduction: Self- assemble structures play an important role in many aspects of nanotechnology field such as biosesnsors, drug delivery, tissue engineering and targeting activity. S-layers proteins are an example of such molecules. These molecules possess the ability of re-cryst...
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University of Isfahan
2015-06-01
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doaj-503158c1bf944a22bce35c8dcdb19ea62020-11-25T00:32:54ZengUniversity of IsfahanBiological Journal of Microorganism2322-51732322-51812015-06-014135766Isolation of S-Layer from Caulobacter and re-crystallization on planar supportsNeda Habibi0tayebe zohrabi1 Isfahan University of technology M. Sc. Student of Nanobiotechnology, University of Isfahan, Iran Introduction: Self- assemble structures play an important role in many aspects of nanotechnology field such as biosesnsors, drug delivery, tissue engineering and targeting activity. S-layers proteins are an example of such molecules. These molecules possess the ability of re-crystallization after isolation from the surface of bacteria and therefore could be used for fabrication of assembled functional groups. Materials and methods: To this regard in this work S- Layers were isolated from Caulobacter and were assembled on mica and silicon wafers. S-Layers re-crystallization were characterized by means of AFM. In order to evaluate the production of assembled functional groups Immunological methods were used. Results: S-Layer with molecular weight of 96 KDa were successfully isolated from the surface of Caulobacter and crystallized on solid silicone and mica support. AFM results indicated assembled domains on mica and silicone. IgG immobilization was induced in the presence of S-Layer structures. Discussion and conclusion: The results confirmed the effect of S-Layer crystallization on fabrication of assembled functional groups.http://uijs.ui.ac.ir/bjm/browse.php?a_code=A-10-152-1&slc_lang=en&sid=1Self- assembly S- Layer Functional group |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Neda Habibi tayebe zohrabi |
spellingShingle |
Neda Habibi tayebe zohrabi Isolation of S-Layer from Caulobacter and re-crystallization on planar supports Biological Journal of Microorganism Self- assembly S- Layer Functional group |
author_facet |
Neda Habibi tayebe zohrabi |
author_sort |
Neda Habibi |
title |
Isolation of S-Layer from Caulobacter and re-crystallization on planar supports |
title_short |
Isolation of S-Layer from Caulobacter and re-crystallization on planar supports |
title_full |
Isolation of S-Layer from Caulobacter and re-crystallization on planar supports |
title_fullStr |
Isolation of S-Layer from Caulobacter and re-crystallization on planar supports |
title_full_unstemmed |
Isolation of S-Layer from Caulobacter and re-crystallization on planar supports |
title_sort |
isolation of s-layer from caulobacter and re-crystallization on planar supports |
publisher |
University of Isfahan |
series |
Biological Journal of Microorganism |
issn |
2322-5173 2322-5181 |
publishDate |
2015-06-01 |
description |
Introduction: Self- assemble
structures play an important role in many aspects of nanotechnology field such
as biosesnsors, drug delivery, tissue engineering and targeting activity.
S-layers proteins are an example of such molecules. These molecules possess the
ability of re-crystallization after isolation from the surface of bacteria and
therefore could be used for fabrication of assembled functional groups. Materials and methods: To this regard in this work S- Layers were isolated from Caulobacter and
were assembled on mica and silicon wafers. S-Layers re-crystallization were
characterized by means of AFM. In order to evaluate the production of assembled
functional groups Immunological methods were used. Results: S-Layer with molecular weight of 96 KDa were successfully isolated from the
surface of Caulobacter and crystallized on solid silicone and mica
support. AFM results indicated assembled domains on mica and silicone. IgG
immobilization was induced in the presence of S-Layer structures. Discussion and conclusion: The results confirmed the effect of S-Layer
crystallization on fabrication of assembled functional groups. |
topic |
Self- assembly S- Layer Functional group |
url |
http://uijs.ui.ac.ir/bjm/browse.php?a_code=A-10-152-1&slc_lang=en&sid=1 |
work_keys_str_mv |
AT nedahabibi isolationofslayerfromcaulobacterandrecrystallizationonplanarsupports AT tayebezohrabi isolationofslayerfromcaulobacterandrecrystallizationonplanarsupports |
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1725318496333594624 |