The influence of low-molecular fraction from cord blood (below 5 kDa) on functional and biochemical parameters of cells in vitro

• Main Authors: A. K. Gulevsky, N. N. Moisieieva, O. L. Gorina, J. S. Akhatova, A. A. Lavrik, A. V. Trifonova
• Format: Article
• Language: English
• Published: National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. 2014-12-01
• Series: Ukrainian Biochemical Journal
• Subjects: Actovegin®; alkaline phosphatase; BHK-21 clone 13/04; cord blood low-molecular fraction; glucose; human embryonic fibroblasts; inhibitors of phagocytosis; leukocytes; mesenchymal stromal cells; PK-15 cells
• Online Access: http://ukrbiochemjournal.org/wp-content/uploads/2015/07/Gulevsky_6_14.pdf

The influence of a low-molecular fraction (below 5 kDa) from the cattle cord blood (CBF) on functional activity of phagocytes, human embryonic fibroblasts, mesenchymal stromal cells and BHK-21 clone 13/04 and PK-15 cells was studied. The low-molecular fraction added to culture medium increases the growth rate of cell cultures. The incubation of leukoconcentrate in the CBF-containing medium results in an increase in phagocytic indices of neutrophils in the presence of a phagocytosis inhibitor – sodium iodoacetate, leading to a significant increase in intracellular glucose content and alkaline phosphatase activity as compared to the control and the reference drug Actovegin®.