Analyses of the Root-Knot Nematode (<i>Meloidogyne graminicola</i>) Transcriptome during Host Infection Highlight Specific Gene Expression Profiling in Resistant Rice Plants

Analyses of the Root-Knot Nematode (<i>Meloidogyne graminicola</i>) Transcriptome during Host Infection Highlight Specific Gene Expression Profiling in Resistant Rice Plants

The plant-parasitic nematode <i>Meloidogyne graminicola</i> causes considerable damages to rice (<i>Oryza sativa</i>) culture. Resistance to <i>M. graminicola</i> in the related species <i>Oryza glaberrima</i> reduces root penetration by juveniles and...

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Main Authors: Anne-Sophie Petitot, Alexis Dereeper, Corinne Da Silva, Julie Guy, Diana Fernandez
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:Pathogens
Subjects:
plant-parasitic nematode
dual RNA-Seq
resistance
virulence effector
cell wall-degrading enzymes
cuticle
Online Access:https://www.mdpi.com/2076-0817/9/8/644
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spelling doaj-4fdc0cbfc7294a2cb3e386ef96ec02ba2020-11-25T03:19:34ZengMDPI AGPathogens2076-08172020-08-01964464410.3390/pathogens9080644Analyses of the Root-Knot Nematode (<i>Meloidogyne graminicola</i>) Transcriptome during Host Infection Highlight Specific Gene Expression Profiling in Resistant Rice PlantsAnne-Sophie Petitot0Alexis Dereeper1Corinne Da Silva2Julie Guy3Diana Fernandez4IRD, Cirad, Univ Montpellier, IPME, 911 Avenue Agropolis, BP 64501, CEDEX 5, 34394 Montpellier, FranceIRD, Cirad, Univ Montpellier, IPME, 911 Avenue Agropolis, BP 64501, CEDEX 5, 34394 Montpellier, FranceGénomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-Saclay, 91057 Evry, FranceGénomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-Saclay, 91057 Evry, FranceIRD, Cirad, Univ Montpellier, IPME, 911 Avenue Agropolis, BP 64501, CEDEX 5, 34394 Montpellier, FranceThe plant-parasitic nematode <i>Meloidogyne graminicola</i> causes considerable damages to rice (<i>Oryza sativa</i>) culture. Resistance to <i>M. graminicola</i> in the related species <i>Oryza glaberrima</i> reduces root penetration by juveniles and stops further nematode development. <i>M. graminicola</i> genes expressed during <i>O. sativa</i> infection were previously characterized but no information is available about the molecular dialogue established with a resistant plant. We compared the <i>M. graminicola</i> transcriptomes of stage-two juveniles (J2s) before and during infection of susceptible or resistant rice. Among 36,121 <i>M. graminicola</i> genes surveyed, 367 were differentially expressed during infection of resistant or susceptible plants. Genes encoding cell wall-degrading enzymes, peptidases and neuropeptides were expressed for a longer time in resistant plants compared to susceptible plants. Conversely, genes related to nematode development were not activated in the resistant host. The majority of <i>M. graminicola</i> effector genes had similar expression patterns, whatever the host genotype. However, two venom allergen-like protein (VAP)-encoding genes were specifically induced in resistant plants and <i>Mg-VAP1</i> silencing in J2s reduced their ability to colonize roots. This study highlighted that <i>M. graminicola</i> adapts its gene expression to the host susceptibility. Further investigation is required to assess the role of <i>Mg-VAPs</i> in the rice–nematode interaction.https://www.mdpi.com/2076-0817/9/8/644plant-parasitic nematodedual RNA-Seqresistancevirulence effectorcell wall-degrading enzymescuticle
collection DOAJ
language English
format Article
sources DOAJ
author Anne-Sophie Petitot
Alexis Dereeper
Corinne Da Silva
Julie Guy
Diana Fernandez
spellingShingle Anne-Sophie Petitot
Alexis Dereeper
Corinne Da Silva
Julie Guy
Diana Fernandez
Analyses of the Root-Knot Nematode (<i>Meloidogyne graminicola</i>) Transcriptome during Host Infection Highlight Specific Gene Expression Profiling in Resistant Rice Plants
Pathogens
plant-parasitic nematode
dual RNA-Seq
resistance
virulence effector
cell wall-degrading enzymes
cuticle
author_facet Anne-Sophie Petitot
Alexis Dereeper
Corinne Da Silva
Julie Guy
Diana Fernandez
author_sort Anne-Sophie Petitot
title Analyses of the Root-Knot Nematode (<i>Meloidogyne graminicola</i>) Transcriptome during Host Infection Highlight Specific Gene Expression Profiling in Resistant Rice Plants
title_short Analyses of the Root-Knot Nematode (<i>Meloidogyne graminicola</i>) Transcriptome during Host Infection Highlight Specific Gene Expression Profiling in Resistant Rice Plants
title_full Analyses of the Root-Knot Nematode (<i>Meloidogyne graminicola</i>) Transcriptome during Host Infection Highlight Specific Gene Expression Profiling in Resistant Rice Plants
title_fullStr Analyses of the Root-Knot Nematode (<i>Meloidogyne graminicola</i>) Transcriptome during Host Infection Highlight Specific Gene Expression Profiling in Resistant Rice Plants
title_full_unstemmed Analyses of the Root-Knot Nematode (<i>Meloidogyne graminicola</i>) Transcriptome during Host Infection Highlight Specific Gene Expression Profiling in Resistant Rice Plants
title_sort analyses of the root-knot nematode (<i>meloidogyne graminicola</i>) transcriptome during host infection highlight specific gene expression profiling in resistant rice plants
publisher MDPI AG
series Pathogens
issn 2076-0817
publishDate 2020-08-01
description The plant-parasitic nematode <i>Meloidogyne graminicola</i> causes considerable damages to rice (<i>Oryza sativa</i>) culture. Resistance to <i>M. graminicola</i> in the related species <i>Oryza glaberrima</i> reduces root penetration by juveniles and stops further nematode development. <i>M. graminicola</i> genes expressed during <i>O. sativa</i> infection were previously characterized but no information is available about the molecular dialogue established with a resistant plant. We compared the <i>M. graminicola</i> transcriptomes of stage-two juveniles (J2s) before and during infection of susceptible or resistant rice. Among 36,121 <i>M. graminicola</i> genes surveyed, 367 were differentially expressed during infection of resistant or susceptible plants. Genes encoding cell wall-degrading enzymes, peptidases and neuropeptides were expressed for a longer time in resistant plants compared to susceptible plants. Conversely, genes related to nematode development were not activated in the resistant host. The majority of <i>M. graminicola</i> effector genes had similar expression patterns, whatever the host genotype. However, two venom allergen-like protein (VAP)-encoding genes were specifically induced in resistant plants and <i>Mg-VAP1</i> silencing in J2s reduced their ability to colonize roots. This study highlighted that <i>M. graminicola</i> adapts its gene expression to the host susceptibility. Further investigation is required to assess the role of <i>Mg-VAPs</i> in the rice–nematode interaction.
topic plant-parasitic nematode
dual RNA-Seq
resistance
virulence effector
cell wall-degrading enzymes
cuticle
url https://www.mdpi.com/2076-0817/9/8/644
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AT corinnedasilva analysesoftherootknotnematodeimeloidogynegraminicolaitranscriptomeduringhostinfectionhighlightspecificgeneexpressionprofilinginresistantriceplants
AT julieguy analysesoftherootknotnematodeimeloidogynegraminicolaitranscriptomeduringhostinfectionhighlightspecificgeneexpressionprofilinginresistantriceplants
AT dianafernandez analysesoftherootknotnematodeimeloidogynegraminicolaitranscriptomeduringhostinfectionhighlightspecificgeneexpressionprofilinginresistantriceplants
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