Infectious bronchitis virus: detection and vaccine Strain differentiation by semi-nested RT-PCR

Infectious bronchitis virus: detection and vaccine Strain differentiation by semi-nested RT-PCR

A semi-nested reverse transcription-polymerase chain reaction (Semi-N-RT-PCR) was developed and used to detect the S glycoprotein gene of infectious bronchitis virus (IBV) strains and to discriminate H120 vaccine strain from other strains. Viral RNA was extracted from the allantoic fluid of chicken...

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Bibliographic Details
Main Authors:	CH Okino, MFSM Montassier, PEN Givisiez, CRAG Furuyama, L Brentano, HJ Montassier
Format:	Article
Language:	English
Published:	Fundação APINCO de Ciência e Tecnologia Avícolas 2005-03-01
Series:	Brazilian Journal of Poultry Science
Subjects:	detection differentiation infectious bronchitis virus semi-nested RT-PCR vaccine strain
Online Access:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2005000100010

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