The effect of aqueous extract of Rosa damascena on formaldehyde-induced toxicity in mice testes

• Main Authors: Majid Askaripour, Azam Hasanpour, Foruzan Hosseini, Mojgan Moshrefi, Gholamreza Moshtaghi, Mohammad Hasannejad, Soodeh Rajabi, Seyed Noureddin Nematollahi-Mahani
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2018-01-01
• Series: Pharmaceutical Biology
• Subjects: formaldehyde toxicity; oxidative stress; antioxidant
• Online Access: http://dx.doi.org/10.1080/13880209.2017.1413663
• ISSN: 1388-0209; 1744-5116

Context: Rosa damascena L. (Rosaceae) (RD) essential oil and extracts are commonly used as a flavour in herbal medicine which increase libido. Previous studies have shown inhalation of RD flower’s oil increases libido and causes protective effects in formaldehyde (FA)-induced testicular damage. Objective: The protective effects of aqueous extract of RD on the male reproductive system of mice were examined following FA-induced damage. Materials and methods: Forty-eight adult NMRI male mice were randomly assigned to six groups (n = 8): control (normal saline, 10 mg/kg); RD40 (40 mg/kg, p.o.); FA treated (10 mg/kg of 10%, i.p.) and FA + RD treated at 10, 20 and 40 mg/kg (FA + RD10), (FA + RD20) and (FA + RD40), respectively, for 40 days. At the end of treatment regimes, serum testosterone (T) level and the reproductive activity, viz. body/organ weights, testicular structure and sperm characteristics were studied. Results: Formaldehyde administration significantly decreased serum T level (p < 0.001), testicular weight/volume, tubular diameter and sperm characteristics compared to the control group (p < 0.05). RD (40 mg/kg) administration in FA-treated mice significantly improved serum T level, testicular weight/histological structure, tubular diameter, Leydig cell number and epididymal sperm characteristics in comparison to its lower doses and the control group (p < 0.05). Discussion and conclusions: We may conclude that RD flower extract can withstand effects of FA in the male reproductive system of mice possibly due to its antioxidative properties.