Validation of an Analytical Method for the Simultaneous Determination of Hyaluronic Acid Concentration and Molecular Weight by Size-Exclusion Chromatography

• Main Authors: Luis Antonio Suárez-Hernández, Rosa María Camacho-Ruíz, Enrique Arriola-Guevara, Eduardo Padilla-Camberos, Manuel Reinhart Kirchmayr, Rosa Isela Corona-González, Guadalupe María Guatemala-Morales
• Format: Article
• Language: English
• Published: MDPI AG 2021-09-01
• Series: Molecules
• Subjects: hyaluronic acid; size-exclusion chromatography; molecular weight distribution; analytical method; <i>Streptococcus zooepidemicus</i>
• Online Access: https://www.mdpi.com/1420-3049/26/17/5360

The hyaluronic acid (HA) global market growth can be attributed to its use in medical, cosmetic, and pharmaceutical applications; thus, it is important to have validated, analytical methods to ensure confidence and security of its use (and to save time and resources). In this work, a size-exclusion chromatography method (HPLC-SEC) was validated to determine the concentration and molecular distribution of HA simultaneously. Analytical curves were developed for concentration and molecular weight in the ranges of 100–1000 mg/L and 0.011–2.200 MDa, respectively. The HPLC-SEC method showed repeatability and reproducibility greater than 98% and limits of detection and quantification of 12 and 42 mg/L, respectively, and was successfully applied to the analysis of HA from a bacterial culture, as well as cosmetic, and pharmaceutical products.