Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-Palindromic NFAT-Response Elements Facilitates Analysis of Biological Endpoint Effects of Cellular Ca2+ Mobilization

Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-Palindromic NFAT-Response Elements Facilitates Analysis of Biological Endpoint Effects of Cellular Ca2+ Mobilization

NFAT is a cytoplasm-localized hyper-phosphorylated transcription factor that is activated through dephosphorylation by calcineurin, a Ca2+/calmodulin-dependent phosphatase. A non-palindromic NFAT-response element (RE) found in the IL2 promoter region has been commonly used for a Ca2+-response report...

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Bibliographic Details
Main Authors: Wei Zhang, Terunao Takahara, Takuya Achiha, Hideki Shibata, Masatoshi Maki
Format: Article
Language:English
Published: MDPI AG 2018-02-01
Series:International Journal of Molecular Sciences
Subjects:
ALG-2
calcium-binding protein
calcineurin
calcium signaling
carbachol
nanoluciferase
NFAT
PDCD6
reporter gene assay
SOCE
transcription factor
Online Access:http://www.mdpi.com/1422-0067/19/2/605

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