Transcriptomic Analysis Reveals the Molecular Adaptation of Three Major Secondary Metabolic Pathways to Multiple Macronutrient Starvation in Tea (<i>Camellia sinensis</i>)

Transcriptomic Analysis Reveals the Molecular Adaptation of Three Major Secondary Metabolic Pathways to Multiple Macronutrient Starvation in Tea (<i>Camellia sinensis</i>)

Tea (<i>Camellia sinensis</i> (L.) O. Kuntze) is a widely consumed beverage. Lack of macronutrients is a major cause of tea yield and quality losses. Though the effects of macronutrient starvation on tea metabolism have been studied, little is known about their molecular mechanisms. Henc...

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Main Authors: Hui Su, Xueying Zhang, Yuqing He, Linying Li, Yuefei Wang, Gaojie Hong, Ping Xu
Format: Article
Language:English
Published: MDPI AG 2020-02-01
Series:Genes
Subjects:
<i>camellia sinensis</i>
transcriptomics
macronutrient starvation
secondary metabolite
transcription factors
Online Access:https://www.mdpi.com/2073-4425/11/3/241
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spelling doaj-4f281df0c465484cb955b207e3ecebf42020-11-25T01:55:08ZengMDPI AGGenes2073-44252020-02-0111324110.3390/genes11030241genes11030241Transcriptomic Analysis Reveals the Molecular Adaptation of Three Major Secondary Metabolic Pathways to Multiple Macronutrient Starvation in Tea (<i>Camellia sinensis</i>)Hui Su0Xueying Zhang1Yuqing He2Linying Li3Yuefei Wang4Gaojie Hong5Ping Xu6Department of Tea Science, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory for Quality and Safety of Agro-products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Quality and Safety of Agro-products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Quality and Safety of Agro-products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaDepartment of Tea Science, Zhejiang University, Hangzhou 310058, ChinaState Key Laboratory for Quality and Safety of Agro-products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaDepartment of Tea Science, Zhejiang University, Hangzhou 310058, ChinaTea (<i>Camellia sinensis</i> (L.) O. Kuntze) is a widely consumed beverage. Lack of macronutrients is a major cause of tea yield and quality losses. Though the effects of macronutrient starvation on tea metabolism have been studied, little is known about their molecular mechanisms. Hence, we investigated changes in the gene expression of tea plants under nitrogen (N), phosphate (P), and potassium (K) deficient conditions by RNA-sequencing. A total of 9103 differentially expressed genes (DEG) were identified. Function enrichment analysis showed that many biological processes and pathways were common to N, P, and K starvation. In particular, cis-element analysis of promoter of genes uncovered that members of the WRKY, MYB, bHLH, NF-Y, NAC, Trihelix, and GATA families were more likely to regulate genes involved in catechins, <span style="font-variant: small-caps;">l</span>-theanine, and caffeine biosynthetic pathways. Our results provide a comprehensive insight into the mechanisms of responses to N, P, and K starvation, and a global basis for the improvement of tea quality and molecular breeding.https://www.mdpi.com/2073-4425/11/3/241<i>camellia sinensis</i>transcriptomicsmacronutrient starvationsecondary metabolitetranscription factors
collection DOAJ
language English
format Article
sources DOAJ
author Hui Su
Xueying Zhang
Yuqing He
Linying Li
Yuefei Wang
Gaojie Hong
Ping Xu
spellingShingle Hui Su
Xueying Zhang
Yuqing He
Linying Li
Yuefei Wang
Gaojie Hong
Ping Xu
Transcriptomic Analysis Reveals the Molecular Adaptation of Three Major Secondary Metabolic Pathways to Multiple Macronutrient Starvation in Tea (<i>Camellia sinensis</i>)
Genes
<i>camellia sinensis</i>
transcriptomics
macronutrient starvation
secondary metabolite
transcription factors
author_facet Hui Su
Xueying Zhang
Yuqing He
Linying Li
Yuefei Wang
Gaojie Hong
Ping Xu
author_sort Hui Su
title Transcriptomic Analysis Reveals the Molecular Adaptation of Three Major Secondary Metabolic Pathways to Multiple Macronutrient Starvation in Tea (<i>Camellia sinensis</i>)
title_short Transcriptomic Analysis Reveals the Molecular Adaptation of Three Major Secondary Metabolic Pathways to Multiple Macronutrient Starvation in Tea (<i>Camellia sinensis</i>)
title_full Transcriptomic Analysis Reveals the Molecular Adaptation of Three Major Secondary Metabolic Pathways to Multiple Macronutrient Starvation in Tea (<i>Camellia sinensis</i>)
title_fullStr Transcriptomic Analysis Reveals the Molecular Adaptation of Three Major Secondary Metabolic Pathways to Multiple Macronutrient Starvation in Tea (<i>Camellia sinensis</i>)
title_full_unstemmed Transcriptomic Analysis Reveals the Molecular Adaptation of Three Major Secondary Metabolic Pathways to Multiple Macronutrient Starvation in Tea (<i>Camellia sinensis</i>)
title_sort transcriptomic analysis reveals the molecular adaptation of three major secondary metabolic pathways to multiple macronutrient starvation in tea (<i>camellia sinensis</i>)
publisher MDPI AG
series Genes
issn 2073-4425
publishDate 2020-02-01
description Tea (<i>Camellia sinensis</i> (L.) O. Kuntze) is a widely consumed beverage. Lack of macronutrients is a major cause of tea yield and quality losses. Though the effects of macronutrient starvation on tea metabolism have been studied, little is known about their molecular mechanisms. Hence, we investigated changes in the gene expression of tea plants under nitrogen (N), phosphate (P), and potassium (K) deficient conditions by RNA-sequencing. A total of 9103 differentially expressed genes (DEG) were identified. Function enrichment analysis showed that many biological processes and pathways were common to N, P, and K starvation. In particular, cis-element analysis of promoter of genes uncovered that members of the WRKY, MYB, bHLH, NF-Y, NAC, Trihelix, and GATA families were more likely to regulate genes involved in catechins, <span style="font-variant: small-caps;">l</span>-theanine, and caffeine biosynthetic pathways. Our results provide a comprehensive insight into the mechanisms of responses to N, P, and K starvation, and a global basis for the improvement of tea quality and molecular breeding.
topic <i>camellia sinensis</i>
transcriptomics
macronutrient starvation
secondary metabolite
transcription factors
url https://www.mdpi.com/2073-4425/11/3/241
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