Spatial Modelling of Bacterial Diversity over the Selected Regions in Bangladesh by Next-Generation Sequencing: Role of Water Temperature

• Main Authors: Nabila Akter, Md Wahiduzzaman, Alea Yeasmin, Kazi Saiful Islam, Jing-Jia Luo
• Format: Article
• Language: English
• Published: MDPI AG 2020-04-01
• Series: Applied Sciences
• Subjects: spatial modelling; bacterial distribution; next generation sequencing; water temperature; Bangladesh
• Online Access: https://www.mdpi.com/2076-3417/10/7/2537

In this study, a spatial model has been developed to investigate the role of water temperature to the distribution of bacteria over the selected regions in the Bay of Bengal, located in the southern region of Bangladesh using next-generation sequencing. Bacterial concentration, quantitative polymerase chain reactions, and sequencing were performed on water samples and identified Acidobacteria, Actinobacteria, Bacteroidetes, Chlorobi, Chloroflexi, Cyanobacteria, Firmicutes, Nitrospirae, Planctomycetes, Proteobacteria, and Verrucomicrobia. The spatial model tessellated the parts of the Bay of Bengal with hexagons and analyzed the relationship between the distribution of bacteria and water temperature. A geographically weighted regression was used to observe whether water temperature contributed strongly or weakly to the distribution of bacteria. The residuals were examined to assess the model’s fitness. The spatial model has the potential to predict the bacterial diversity in the selected regions of Bangladesh.