In vitro antimicrobial assessment of Cuban propolis extracts

Propolis is a resinous mixture of different plant exudates collected by honeybees. Currently, propolis is widely used as a food supplement and in folk medicine. We have evaluated 20 Cuban propolis extracts of different chemical types, brown (BCP), red and yellow (YCP), with respect to their in vitro...

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Main Authors: Lianet Monzote, Osmany Cuesta-Rubio, Mercedes Campo Fernandez, Ingrid Márquez Hernandez, Jorge Fraga, Kleich Pérez, Monique Kerstens, Louis Maes, Paul Cos
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2012-12-01
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000800003&lng=en&tlng=en
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spelling doaj-4f26120530b04e9baa101616594b9c262020-11-24T21:42:48ZengInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz.1678-80602012-12-01107897898410.1590/S0074-02762012000800003S0074-02762012000800003In vitro antimicrobial assessment of Cuban propolis extractsLianet Monzote0Osmany Cuesta-Rubio1Mercedes Campo Fernandez2Ingrid Márquez Hernandez3Jorge Fraga4Kleich Pérez5Monique Kerstens6Louis Maes7Paul Cos8Instituto de Medicina Tropical Pedro KouríHavana UniversityHavana UniversityHavana UniversityInstituto de Medicina Tropical Pedro KouríInstituto de Medicina Tropical Pedro KouríAntwerp UniversityAntwerp UniversityAntwerp UniversityPropolis is a resinous mixture of different plant exudates collected by honeybees. Currently, propolis is widely used as a food supplement and in folk medicine. We have evaluated 20 Cuban propolis extracts of different chemical types, brown (BCP), red and yellow (YCP), with respect to their in vitro antibacterial, antifungal and antiprotozoal properties. The extracts inhibited the growth of Staphylococcus aureus and Trichophyton rubrum at low µg/mL concentrations, whereas they were not active against Escherichia coli and Candida albicans. The major activity of the extracts was found against the protozoa Leishmania, Trypanosoma and Plasmodium, although cytotoxicity against MRC-5 cells was also observed. The BCP-3, YCP-39 and YCP-60 extracts showed the highest activity against P. falciparum, with 50% of microbial growth (IC50) values of 0.2 µg/mL. A positive correlation between the biological activity and the chemical composition was observed for YCP extracts. The most promising antimicrobial activity corresponds to YCP subtype B, which contains acetyl triterpenes as the main constituents. The present in vitro study highlights the potential of propolis against protozoa, but further research is needed to increase selectivity towards the parasite. The observed chemical composition-activity relationship of propolis can contribute to the identification of the active principles and standardisation of this bee product.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000800003&lng=en&tlng=enpropolisCubanantibacterialantifungalantiprotozoal
collection DOAJ
language English
format Article
sources DOAJ
author Lianet Monzote
Osmany Cuesta-Rubio
Mercedes Campo Fernandez
Ingrid Márquez Hernandez
Jorge Fraga
Kleich Pérez
Monique Kerstens
Louis Maes
Paul Cos
spellingShingle Lianet Monzote
Osmany Cuesta-Rubio
Mercedes Campo Fernandez
Ingrid Márquez Hernandez
Jorge Fraga
Kleich Pérez
Monique Kerstens
Louis Maes
Paul Cos
In vitro antimicrobial assessment of Cuban propolis extracts
Memórias do Instituto Oswaldo Cruz.
propolis
Cuban
antibacterial
antifungal
antiprotozoal
author_facet Lianet Monzote
Osmany Cuesta-Rubio
Mercedes Campo Fernandez
Ingrid Márquez Hernandez
Jorge Fraga
Kleich Pérez
Monique Kerstens
Louis Maes
Paul Cos
author_sort Lianet Monzote
title In vitro antimicrobial assessment of Cuban propolis extracts
title_short In vitro antimicrobial assessment of Cuban propolis extracts
title_full In vitro antimicrobial assessment of Cuban propolis extracts
title_fullStr In vitro antimicrobial assessment of Cuban propolis extracts
title_full_unstemmed In vitro antimicrobial assessment of Cuban propolis extracts
title_sort in vitro antimicrobial assessment of cuban propolis extracts
publisher Instituto Oswaldo Cruz, Ministério da Saúde
series Memórias do Instituto Oswaldo Cruz.
issn 1678-8060
publishDate 2012-12-01
description Propolis is a resinous mixture of different plant exudates collected by honeybees. Currently, propolis is widely used as a food supplement and in folk medicine. We have evaluated 20 Cuban propolis extracts of different chemical types, brown (BCP), red and yellow (YCP), with respect to their in vitro antibacterial, antifungal and antiprotozoal properties. The extracts inhibited the growth of Staphylococcus aureus and Trichophyton rubrum at low µg/mL concentrations, whereas they were not active against Escherichia coli and Candida albicans. The major activity of the extracts was found against the protozoa Leishmania, Trypanosoma and Plasmodium, although cytotoxicity against MRC-5 cells was also observed. The BCP-3, YCP-39 and YCP-60 extracts showed the highest activity against P. falciparum, with 50% of microbial growth (IC50) values of 0.2 µg/mL. A positive correlation between the biological activity and the chemical composition was observed for YCP extracts. The most promising antimicrobial activity corresponds to YCP subtype B, which contains acetyl triterpenes as the main constituents. The present in vitro study highlights the potential of propolis against protozoa, but further research is needed to increase selectivity towards the parasite. The observed chemical composition-activity relationship of propolis can contribute to the identification of the active principles and standardisation of this bee product.
topic propolis
Cuban
antibacterial
antifungal
antiprotozoal
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000800003&lng=en&tlng=en
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