Aggravated biofouling caused by chlorine disinfection in a pilot-scale reverse osmosis treatment system of municipal wastewater

• Main Authors: Li-Wei Luo, Yin-Hu Wu, Yun-Hong Wang, Xin Tong, Yuan Bai, Gen-Qiang Chen, Hao-Bin Wang, Nozomu Ikuno, Hong-Ying Hu
• Format: Article
• Language: English
• Published: IWA Publishing 2021-06-01
• Series: Water Reuse
• Subjects: biofouling; chlorine disinfection; chlorine-resistant bacteria (crb); reverse osmosis; wastewater reclamation
• Online Access: http://jwrd.iwaponline.com/content/11/2/201
• ISSN: 2709-6092; 2709-6106

The reverse osmosis (RO) system is widely applied to produce reclaimed water for high-standard industrial use. Chlorine disinfection is the main biofouling control method in the RO systems for wastewater reclamation. However, researchers reported the adverse effects of chlorine disinfection which aggravated biofouling in laboratory-scale RO systems. In this study, four parallel 4-inch spiral wound RO membranes were used to study the effect of chlorine on biofouling in a pilot-scale RO system. The free chlorine dosages in four experimental groups were 0, 1, 2 and 5 mg/L, respectively. After continuous chlorination and dechlorination, the feed water entered the RO system. It was found that chlorine pretreatment caused a 1.9–36.7% increase in relative feed water pressure of the RO system, suggesting that chlorine aggravated the membrane fouling in the pilot-scale RO system. The microbial community structures of living bacteria in the feed water of the RO system were determined by the PMA (propidium monoazide)-PCR method and showed that the relative abundance of chlorine-resistant bacteria (CRB) was significantly increased after disinfection. Nine major genera which maintained higher relative abundance in experimental groups with high chlorine dosage were considered as possible key species causing membrane fouling, including Pedobacter, Clostridium and Bradyrhizobium. HIGHLIGHTS Revealed the effects of chlorine disinfection on fouling of a pilot-scale RO system.; Chlorine disinfection aggravated the membrane fouling in the pilot RO system.; Analyzed community structure of residual bacteria after disinfection in feed water.; Identified key chlorine-resistant bacteria (CRB) causing RO membrane fouling.;