A Flow Cytometry Protocol to Estimate DNA Content in the Yellowtail Tetra Astyanax altiparanae

The production of triploid yellowtail tetra Astyanax altiparanae is a key factor to obtain permanently sterile individuals by chromosome set manipulation. Flow cytometric analysis is the main tool for confirmation of the resultant triploids individuals, but very few protocols are specific for A. alt...

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Bibliographic Details
Main Authors: Pedro L. P. Xavier, José A. Senhorini, Matheus Pereira-Santos, Takafumi Fujimoto, Eduardo Shimoda, Luciano A. Silva, Silvio A. dos Santos, George S. Yasui
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-09-01
Series:Frontiers in Genetics
Subjects:
Online Access:http://journal.frontiersin.org/article/10.3389/fgene.2017.00131/full