Dual Recombining-out System for Spatiotemporal Gene Expression in C. elegans

Dual Recombining-out System for Spatiotemporal Gene Expression in C. elegans

Summary: Specific recording, labeling, and spatiotemporal manipulating neurons are essential for neuroscience research. In this study, we developed a tripartite spatiotemporal gene induction system in C. elegans, which is based on the knockout of two transcriptional terminators (stops in short) by t...

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Main Authors: Ming-Hai Ge, Wei Wang, Tai-Hong Wu, Xin Wen, Umar Al-Sheikh, Li-Li Chen, Sheng-Wu Yin, Jing-Jing Wu, Jia-Hao Huang, Qing-Qin He, Hui Liu, Rong Li, Ping-Zhou Wang, Zheng-Xing Wu
Format: Article
Language:English
Published: Elsevier 2020-10-01
Series:iScience
Subjects:
Molecular Biology
Neuroscience
Genetic Engineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004220307598
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record_format Article
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language English
format Article
sources DOAJ
author Ming-Hai Ge
Wei Wang
Tai-Hong Wu
Xin Wen
Umar Al-Sheikh
Li-Li Chen
Sheng-Wu Yin
Jing-Jing Wu
Jia-Hao Huang
Qing-Qin He
Hui Liu
Rong Li
Ping-Zhou Wang
Zheng-Xing Wu
spellingShingle Ming-Hai Ge
Wei Wang
Tai-Hong Wu
Xin Wen
Umar Al-Sheikh
Li-Li Chen
Sheng-Wu Yin
Jing-Jing Wu
Jia-Hao Huang
Qing-Qin He
Hui Liu
Rong Li
Ping-Zhou Wang
Zheng-Xing Wu
Dual Recombining-out System for Spatiotemporal Gene Expression in C. elegans
iScience
Molecular Biology
Neuroscience
Genetic Engineering
author_facet Ming-Hai Ge
Wei Wang
Tai-Hong Wu
Xin Wen
Umar Al-Sheikh
Li-Li Chen
Sheng-Wu Yin
Jing-Jing Wu
Jia-Hao Huang
Qing-Qin He
Hui Liu
Rong Li
Ping-Zhou Wang
Zheng-Xing Wu
author_sort Ming-Hai Ge
title Dual Recombining-out System for Spatiotemporal Gene Expression in C. elegans
title_short Dual Recombining-out System for Spatiotemporal Gene Expression in C. elegans
title_full Dual Recombining-out System for Spatiotemporal Gene Expression in C. elegans
title_fullStr Dual Recombining-out System for Spatiotemporal Gene Expression in C. elegans
title_full_unstemmed Dual Recombining-out System for Spatiotemporal Gene Expression in C. elegans
title_sort dual recombining-out system for spatiotemporal gene expression in c. elegans
publisher Elsevier
series iScience
issn 2589-0042
publishDate 2020-10-01
description Summary: Specific recording, labeling, and spatiotemporal manipulating neurons are essential for neuroscience research. In this study, we developed a tripartite spatiotemporal gene induction system in C. elegans, which is based on the knockout of two transcriptional terminators (stops in short) by two different recombinases FLP and CRE. The recombinase sites (loxP and FRT) flanked stops after a ubiquitous promoter terminate transcription of target genes. FLP and CRE, induced by two promoters of overlapping expression, remove the stops (subsequent FLP/CRE-out). The system provides an ''AND'' gate strategy for specific gene expression in single types of cell(s). Combined with an inducible promoter or element, the system can control the spatiotemporal expression of genes in defined cell types, especially in cells or tissues lacking a specific promoter. This tripartite FLP/CRE-out gene expression system is a simple, labor- and cost-saving toolbox for cell type-specific and inducible gene expression in C. elegans.
topic Molecular Biology
Neuroscience
Genetic Engineering
url http://www.sciencedirect.com/science/article/pii/S2589004220307598
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spelling doaj-4ec72bcc22044cad8e3f336d1aa8a3022020-11-25T03:09:37ZengElsevieriScience2589-00422020-10-012310101567Dual Recombining-out System for Spatiotemporal Gene Expression in C. elegansMing-Hai Ge0Wei Wang1Tai-Hong Wu2Xin Wen3Umar Al-Sheikh4Li-Li Chen5Sheng-Wu Yin6Jing-Jing Wu7Jia-Hao Huang8Qing-Qin He9Hui Liu10Rong Li11Ping-Zhou Wang12Zheng-Xing Wu13Key Laboratory of Molecular Biophysics of Ministry of Education, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaKey Laboratory of Molecular Biophysics of Ministry of Education, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaKey Laboratory of Molecular Biophysics of Ministry of Education, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaKey Laboratory of Molecular Biophysics of Ministry of Education, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaKey Laboratory of Molecular Biophysics of Ministry of Education, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaKey Laboratory of Molecular Biophysics of Ministry of Education, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaKey Laboratory of Molecular Biophysics of Ministry of Education, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaKey Laboratory of Molecular Biophysics of Ministry of Education, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaKey Laboratory of Molecular Biophysics of Ministry of Education, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaKey Laboratory of Molecular Biophysics of Ministry of Education, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaKey Laboratory of Molecular Biophysics of Ministry of Education, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaKey Laboratory of Molecular Biophysics of Ministry of Education, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaKey Laboratory of Molecular Biophysics of Ministry of Education, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, ChinaKey Laboratory of Molecular Biophysics of Ministry of Education, Institute of Biophysics and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China; Corresponding authorSummary: Specific recording, labeling, and spatiotemporal manipulating neurons are essential for neuroscience research. In this study, we developed a tripartite spatiotemporal gene induction system in C. elegans, which is based on the knockout of two transcriptional terminators (stops in short) by two different recombinases FLP and CRE. The recombinase sites (loxP and FRT) flanked stops after a ubiquitous promoter terminate transcription of target genes. FLP and CRE, induced by two promoters of overlapping expression, remove the stops (subsequent FLP/CRE-out). The system provides an ''AND'' gate strategy for specific gene expression in single types of cell(s). Combined with an inducible promoter or element, the system can control the spatiotemporal expression of genes in defined cell types, especially in cells or tissues lacking a specific promoter. This tripartite FLP/CRE-out gene expression system is a simple, labor- and cost-saving toolbox for cell type-specific and inducible gene expression in C. elegans.http://www.sciencedirect.com/science/article/pii/S2589004220307598Molecular BiologyNeuroscienceGenetic Engineering

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