Investigation of factors affecting xylanase activity from Trichoderma harzianum 1073 D3

• Main Authors: Seyis Isil, Aksoz Nilufer
• Format: Article
• Language: English
• Published: Instituto de Tecnologia do Paraná (Tecpar) 2005-03-01
• Series: Brazilian Archives of Biology and Technology
• Subjects: Trichoderma harzianum; xylan; xylanase; lignocellulosic material; enzyme stability; metal ions
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200004

In this study, some physiological conditions affecting the activity of xylanase enzyme produced from Trichoderma harzianum 1073 D3 were determined. In addition, stabilization of pH and temperature in liquid and semi-solid state cultivation media were investigated. It was concluded that for maximum xylanase activity, incubation at 60&deg;C in an enzyme incubation medium with pH 5 that contained 1 % xylan was appropriate. The stability studies showed that the enzyme was relatively stable in the pH range 3-7 and retained more than 50 % of its original activity after four months.<br>Neste estudo algumas condições que afetam a atividade da enzima xylanase produzida a partir de Trichoderma harzianum 1073 D3 foram determinadas. A estabilização do pH e temperatura em cultivo líquido e semi-sólido foram avaliados. Foi concluído que para o máximo de atividade xylanase foi obtida com 1% de xylano a temperatura de 60ºC e pH 5.0. Estudos de estabilidade demonstraram que a enzima foi relativamente estável na faixa de pH entre 3-7 e retém mais do que 50% de sua atividade original após 4 meses.