Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions

Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions

Summary: In the past few years, several different methods for differentiation of directed cardiomyocytes from human pluripotent stem cells (hPSCs) in chemically defined conditions have been reported, including our own (Burridge et al., 2014; Lian et al., 2012; Lin et al., 2017). To help researchers...

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Main Authors: Yongshun Lin, Jizhong Zou
Format: Article
Language:English
Published: Elsevier 2020-06-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720300022
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spelling doaj-4e6e499dfc194996a316d7eef6a464882020-11-25T04:07:01ZengElsevierSTAR Protocols2666-16672020-06-0111100015Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined ConditionsYongshun Lin0Jizhong Zou1iPSC Core, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA; Corresponding authoriPSC Core, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA; Corresponding authorSummary: In the past few years, several different methods for differentiation of directed cardiomyocytes from human pluripotent stem cells (hPSCs) in chemically defined conditions have been reported, including our own (Burridge et al., 2014; Lian et al., 2012; Lin et al., 2017). To help researchers adapt to our simple and cost-effective method, we provide step-by-step protocols for hPSC-cardiomyocyte differentiation, including hPSC culture, cardiomyocyte differentiation, cardiomyocyte passaging, and cryopreservation.For complete details on the use and execution of this protocol, please refer to Lin et al. (2017).http://www.sciencedirect.com/science/article/pii/S2666166720300022
collection DOAJ
language English
format Article
sources DOAJ
author Yongshun Lin
Jizhong Zou
spellingShingle Yongshun Lin
Jizhong Zou
Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions
STAR Protocols
author_facet Yongshun Lin
Jizhong Zou
author_sort Yongshun Lin
title Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions
title_short Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions
title_full Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions
title_fullStr Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions
title_full_unstemmed Differentiation of Cardiomyocytes from Human Pluripotent Stem Cells in Fully Chemically Defined Conditions
title_sort differentiation of cardiomyocytes from human pluripotent stem cells in fully chemically defined conditions
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2020-06-01
description Summary: In the past few years, several different methods for differentiation of directed cardiomyocytes from human pluripotent stem cells (hPSCs) in chemically defined conditions have been reported, including our own (Burridge et al., 2014; Lian et al., 2012; Lin et al., 2017). To help researchers adapt to our simple and cost-effective method, we provide step-by-step protocols for hPSC-cardiomyocyte differentiation, including hPSC culture, cardiomyocyte differentiation, cardiomyocyte passaging, and cryopreservation.For complete details on the use and execution of this protocol, please refer to Lin et al. (2017).
url http://www.sciencedirect.com/science/article/pii/S2666166720300022
work_keys_str_mv AT yongshunlin differentiationofcardiomyocytesfromhumanpluripotentstemcellsinfullychemicallydefinedconditions
AT jizhongzou differentiationofcardiomyocytesfromhumanpluripotentstemcellsinfullychemicallydefinedconditions
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