Neutral fragment filtering for rapid identification of new diester-diterpenoid alkaloids in roots of Aconitum carmichaeli by ultra-high-pressure liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry.

Neutral fragment filtering for rapid identification of new diester-diterpenoid alkaloids in roots of Aconitum carmichaeli by ultra-high-pressure liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry.

A rapid and effective method was developed for separation and identification of diester-diterpenoid alkaloids (DDA) in the roots of Aconitum carmichaeli by ultra-high-pressure liquid chromatography coupled with high resolution LTQ-Orbitrap tandem mass spectrometry (UHPLC-LTQ-Orbitrap-MS(n)). Accordi...

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Main Authors: Jing Zhang, Zhi Hai Huang, Xiao Hui Qiu, Yi Ming Yang, Da Yuan Zhu, Wen Xu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23285005/pdf/?tool=EBI
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spelling doaj-4e6d64882caa400db04ef01e83f47d4e2021-03-03T20:25:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5235210.1371/journal.pone.0052352Neutral fragment filtering for rapid identification of new diester-diterpenoid alkaloids in roots of Aconitum carmichaeli by ultra-high-pressure liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry.Jing ZhangZhi Hai HuangXiao Hui QiuYi Ming YangDa Yuan ZhuWen XuA rapid and effective method was developed for separation and identification of diester-diterpenoid alkaloids (DDA) in the roots of Aconitum carmichaeli by ultra-high-pressure liquid chromatography coupled with high resolution LTQ-Orbitrap tandem mass spectrometry (UHPLC-LTQ-Orbitrap-MS(n)). According to accurate mass measurement and the characteristic neutral loss filtering strategy, a total of 42 diester-diterpenoid alkaloids (DDA) were rapidly detected and characterized or tentatively identified. Meanwhile, the proposed fragmentation pathways and the major diagnostic fragment ions of aconitine, mesaconitine and hypaconitine were investigated to trace DDA derivatives in crude plant extracts. 23 potential new compounds were successfully screened and characterized in Aconitum carmichaeli, including 16 short chain fatty acyls DDA, 4 N-dealkyl DDA and several isomers of aconitine, mesaconitine and hypaconitine.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23285005/pdf/?tool=EBI
collection DOAJ
language English
format Article
sources DOAJ
author Jing Zhang
Zhi Hai Huang
Xiao Hui Qiu
Yi Ming Yang
Da Yuan Zhu
Wen Xu
spellingShingle Jing Zhang
Zhi Hai Huang
Xiao Hui Qiu
Yi Ming Yang
Da Yuan Zhu
Wen Xu
Neutral fragment filtering for rapid identification of new diester-diterpenoid alkaloids in roots of Aconitum carmichaeli by ultra-high-pressure liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry.
PLoS ONE
author_facet Jing Zhang
Zhi Hai Huang
Xiao Hui Qiu
Yi Ming Yang
Da Yuan Zhu
Wen Xu
author_sort Jing Zhang
title Neutral fragment filtering for rapid identification of new diester-diterpenoid alkaloids in roots of Aconitum carmichaeli by ultra-high-pressure liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry.
title_short Neutral fragment filtering for rapid identification of new diester-diterpenoid alkaloids in roots of Aconitum carmichaeli by ultra-high-pressure liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry.
title_full Neutral fragment filtering for rapid identification of new diester-diterpenoid alkaloids in roots of Aconitum carmichaeli by ultra-high-pressure liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry.
title_fullStr Neutral fragment filtering for rapid identification of new diester-diterpenoid alkaloids in roots of Aconitum carmichaeli by ultra-high-pressure liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry.
title_full_unstemmed Neutral fragment filtering for rapid identification of new diester-diterpenoid alkaloids in roots of Aconitum carmichaeli by ultra-high-pressure liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry.
title_sort neutral fragment filtering for rapid identification of new diester-diterpenoid alkaloids in roots of aconitum carmichaeli by ultra-high-pressure liquid chromatography coupled with linear ion trap-orbitrap mass spectrometry.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2012-01-01
description A rapid and effective method was developed for separation and identification of diester-diterpenoid alkaloids (DDA) in the roots of Aconitum carmichaeli by ultra-high-pressure liquid chromatography coupled with high resolution LTQ-Orbitrap tandem mass spectrometry (UHPLC-LTQ-Orbitrap-MS(n)). According to accurate mass measurement and the characteristic neutral loss filtering strategy, a total of 42 diester-diterpenoid alkaloids (DDA) were rapidly detected and characterized or tentatively identified. Meanwhile, the proposed fragmentation pathways and the major diagnostic fragment ions of aconitine, mesaconitine and hypaconitine were investigated to trace DDA derivatives in crude plant extracts. 23 potential new compounds were successfully screened and characterized in Aconitum carmichaeli, including 16 short chain fatty acyls DDA, 4 N-dealkyl DDA and several isomers of aconitine, mesaconitine and hypaconitine.
url https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23285005/pdf/?tool=EBI
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AT zhihaihuang neutralfragmentfilteringforrapididentificationofnewdiesterditerpenoidalkaloidsinrootsofaconitumcarmichaelibyultrahighpressureliquidchromatographycoupledwithlineariontraporbitrapmassspectrometry
AT xiaohuiqiu neutralfragmentfilteringforrapididentificationofnewdiesterditerpenoidalkaloidsinrootsofaconitumcarmichaelibyultrahighpressureliquidchromatographycoupledwithlineariontraporbitrapmassspectrometry
AT yimingyang neutralfragmentfilteringforrapididentificationofnewdiesterditerpenoidalkaloidsinrootsofaconitumcarmichaelibyultrahighpressureliquidchromatographycoupledwithlineariontraporbitrapmassspectrometry
AT dayuanzhu neutralfragmentfilteringforrapididentificationofnewdiesterditerpenoidalkaloidsinrootsofaconitumcarmichaelibyultrahighpressureliquidchromatographycoupledwithlineariontraporbitrapmassspectrometry
AT wenxu neutralfragmentfilteringforrapididentificationofnewdiesterditerpenoidalkaloidsinrootsofaconitumcarmichaelibyultrahighpressureliquidchromatographycoupledwithlineariontraporbitrapmassspectrometry
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