Genome Sequencing of Streptomyces atratus SCSIOZH16 and Activation Production of Nocardamine via Metabolic Engineering

Genome Sequencing of Streptomyces atratus SCSIOZH16 and Activation Production of Nocardamine via Metabolic Engineering

The Actinomycetes are metabolically flexible microorganisms capable of producing a wide range of interesting compounds, including but by no means limited to, siderophores which have high affinity for ferric iron. In this study, we report the complete genome sequence of marine-derived Streptomyces at...

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Main Authors: Yan Li, Chunyan Zhang, Chengxiong Liu, Jianhua Ju, Junying Ma
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-06-01
Series:Frontiers in Microbiology
Subjects:
Streptomyces atratus ZH16
in-frame deletion
metabolic engineering
siderophore
nocardamine
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2018.01269/full
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spelling doaj-4e300bf75e7b40f6859ee9de46d51ea22020-11-24T23:30:56ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-06-01910.3389/fmicb.2018.01269381939Genome Sequencing of Streptomyces atratus SCSIOZH16 and Activation Production of Nocardamine via Metabolic EngineeringYan Li0Yan Li1Chunyan Zhang2Chunyan Zhang3Chengxiong Liu4Jianhua Ju5Jianhua Ju6Junying Ma7Junying Ma8CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaHubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaThe Actinomycetes are metabolically flexible microorganisms capable of producing a wide range of interesting compounds, including but by no means limited to, siderophores which have high affinity for ferric iron. In this study, we report the complete genome sequence of marine-derived Streptomyces atratus ZH16 and the activation of an embedded siderophore gene cluster via the application of metabolic engineering methods. The S. atratus ZH16 genome reveals that this strain has the potential to produce 26 categories of natural products (NPs) barring the ilamycins. Our activation studies revealed S. atratus SCSIO ZH16 to be a promising source of the production of nocardamine-type (desferrioxamine) compounds which are important in treating acute iron intoxication and performing ecological remediation. We conclude that metabolic engineering provides a highly effective strategy by which to discover drug-like compounds and new NPs in the genomic era.https://www.frontiersin.org/article/10.3389/fmicb.2018.01269/fullStreptomyces atratus ZH16in-frame deletionmetabolic engineeringsiderophorenocardamine
collection DOAJ
language English
format Article
sources DOAJ
author Yan Li
Yan Li
Chunyan Zhang
Chunyan Zhang
Chengxiong Liu
Jianhua Ju
Jianhua Ju
Junying Ma
Junying Ma
spellingShingle Yan Li
Yan Li
Chunyan Zhang
Chunyan Zhang
Chengxiong Liu
Jianhua Ju
Jianhua Ju
Junying Ma
Junying Ma
Genome Sequencing of Streptomyces atratus SCSIOZH16 and Activation Production of Nocardamine via Metabolic Engineering
Frontiers in Microbiology
Streptomyces atratus ZH16
in-frame deletion
metabolic engineering
siderophore
nocardamine
author_facet Yan Li
Yan Li
Chunyan Zhang
Chunyan Zhang
Chengxiong Liu
Jianhua Ju
Jianhua Ju
Junying Ma
Junying Ma
author_sort Yan Li
title Genome Sequencing of Streptomyces atratus SCSIOZH16 and Activation Production of Nocardamine via Metabolic Engineering
title_short Genome Sequencing of Streptomyces atratus SCSIOZH16 and Activation Production of Nocardamine via Metabolic Engineering
title_full Genome Sequencing of Streptomyces atratus SCSIOZH16 and Activation Production of Nocardamine via Metabolic Engineering
title_fullStr Genome Sequencing of Streptomyces atratus SCSIOZH16 and Activation Production of Nocardamine via Metabolic Engineering
title_full_unstemmed Genome Sequencing of Streptomyces atratus SCSIOZH16 and Activation Production of Nocardamine via Metabolic Engineering
title_sort genome sequencing of streptomyces atratus scsiozh16 and activation production of nocardamine via metabolic engineering
publisher Frontiers Media S.A.
series Frontiers in Microbiology
issn 1664-302X
publishDate 2018-06-01
description The Actinomycetes are metabolically flexible microorganisms capable of producing a wide range of interesting compounds, including but by no means limited to, siderophores which have high affinity for ferric iron. In this study, we report the complete genome sequence of marine-derived Streptomyces atratus ZH16 and the activation of an embedded siderophore gene cluster via the application of metabolic engineering methods. The S. atratus ZH16 genome reveals that this strain has the potential to produce 26 categories of natural products (NPs) barring the ilamycins. Our activation studies revealed S. atratus SCSIO ZH16 to be a promising source of the production of nocardamine-type (desferrioxamine) compounds which are important in treating acute iron intoxication and performing ecological remediation. We conclude that metabolic engineering provides a highly effective strategy by which to discover drug-like compounds and new NPs in the genomic era.
topic Streptomyces atratus ZH16
in-frame deletion
metabolic engineering
siderophore
nocardamine
url https://www.frontiersin.org/article/10.3389/fmicb.2018.01269/full
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