Validation of Tuba1a as Appropriate Internal Control for Normalization of Gene Expression Analysis during Mouse Lung Development

Validation of Tuba1a as Appropriate Internal Control for Normalization of Gene Expression Analysis during Mouse Lung Development

The expression ratio between the analysed gene and an internal control gene is the most widely used normalization method for quantitative RT-PCR (qRT-PCR) expression analysis. The ideal reference gene for a specific experiment is the one whose expression is not affected by the different experimental...

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Bibliographic Details
Main Authors: Aditi Mehta, Stephanie Dobersch, Reinhard H. Dammann, Saverio Bellusci, Olga N. Ilinskaya, Thomas Braun, Guillermo Barreto
Format: Article
Language:English
Published: MDPI AG 2015-02-01
Series:International Journal of Molecular Sciences
Subjects:
mouse lung development
reference gene
qRT-PCR
geNorm
alpha 1A tubulin
Tuba1a
Online Access:http://www.mdpi.com/1422-0067/16/3/4492

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