Unilocular fat cells in three-dimensional collagen gel matrix culture.

Unilocular fat cells in three-dimensional collagen gel matrix culture.

Three-dimensional culture with collagen gel, developed recently for the in vitro study of some mammalian cells in a more physiological condition than a monolayer culture, was applied for a biological study of unilocular fat cells. Successfully embedded in the gel, the unilocular fat cells were shown...

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Main Authors: H Sugihara, N Yonemitsu, S Toda, S Miyabara, S Funatsumaru, T Matsumoto
Format: Article
Language:English
Published: Elsevier 1988-05-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S002222752038514X
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spelling doaj-4dfca4037cf344a4bd18aede872884862021-04-25T04:19:37ZengElsevierJournal of Lipid Research0022-22751988-05-01295691697Unilocular fat cells in three-dimensional collagen gel matrix culture.H Sugihara0N Yonemitsu1S Toda2S Miyabara3S Funatsumaru4T Matsumoto5Department of Pathology, Saga Medical School, Japan.Department of Pathology, Saga Medical School, Japan.Department of Pathology, Saga Medical School, Japan.Department of Pathology, Saga Medical School, Japan.Department of Pathology, Saga Medical School, Japan.Department of Pathology, Saga Medical School, Japan.Three-dimensional culture with collagen gel, developed recently for the in vitro study of some mammalian cells in a more physiological condition than a monolayer culture, was applied for a biological study of unilocular fat cells. Successfully embedded in the gel, the unilocular fat cells were shown to be able to keep their cellular function and actively proliferate. These findings confirm that unilocular fat cells do undergo proliferation under in vitro conditions as demonstrated in monolayer culture.http://www.sciencedirect.com/science/article/pii/S002222752038514X
collection DOAJ
language English
format Article
sources DOAJ
author H Sugihara
N Yonemitsu
S Toda
S Miyabara
S Funatsumaru
T Matsumoto
spellingShingle H Sugihara
N Yonemitsu
S Toda
S Miyabara
S Funatsumaru
T Matsumoto
Unilocular fat cells in three-dimensional collagen gel matrix culture.
Journal of Lipid Research
author_facet H Sugihara
N Yonemitsu
S Toda
S Miyabara
S Funatsumaru
T Matsumoto
author_sort H Sugihara
title Unilocular fat cells in three-dimensional collagen gel matrix culture.
title_short Unilocular fat cells in three-dimensional collagen gel matrix culture.
title_full Unilocular fat cells in three-dimensional collagen gel matrix culture.
title_fullStr Unilocular fat cells in three-dimensional collagen gel matrix culture.
title_full_unstemmed Unilocular fat cells in three-dimensional collagen gel matrix culture.
title_sort unilocular fat cells in three-dimensional collagen gel matrix culture.
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1988-05-01
description Three-dimensional culture with collagen gel, developed recently for the in vitro study of some mammalian cells in a more physiological condition than a monolayer culture, was applied for a biological study of unilocular fat cells. Successfully embedded in the gel, the unilocular fat cells were shown to be able to keep their cellular function and actively proliferate. These findings confirm that unilocular fat cells do undergo proliferation under in vitro conditions as demonstrated in monolayer culture.
url http://www.sciencedirect.com/science/article/pii/S002222752038514X
work_keys_str_mv AT hsugihara unilocularfatcellsinthreedimensionalcollagengelmatrixculture
AT nyonemitsu unilocularfatcellsinthreedimensionalcollagengelmatrixculture
AT stoda unilocularfatcellsinthreedimensionalcollagengelmatrixculture
AT smiyabara unilocularfatcellsinthreedimensionalcollagengelmatrixculture
AT sfunatsumaru unilocularfatcellsinthreedimensionalcollagengelmatrixculture
AT tmatsumoto unilocularfatcellsinthreedimensionalcollagengelmatrixculture
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