Identification and characterization of sex-linked proteins of Schistosoma mansoni

• Main Authors: A. Maldonado Junior, Giovanni de Simone
• Format: Article
• Language: English
• Published: Instituto Oswaldo Cruz, Ministério da Saúde 1991-03-01
• Series: Memórias do Instituto Oswaldo Cruz.
• Subjects: Schistosoma mansoni; BH and RJ isolates; amphiphilic proteins; tegumental proteins; Triton X-114; sex-linked proteins
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761991000100006

The proteins of adults worms (male and female) of two isolates (BH and RJ) of Shistosoma mansoni were extracted using Triton X-114 phase separation. The SDS-polyacrilamide gel electrophoresis profiles of the three phases (detergent, aqueous and insoluble proteins) obtained were compared after Coomassie blue and silver staining, surface radioiodination and Western blotting. No major differences were detected between the 2 isolates. Of the 25 or more proteins which partitioned into the detergent phase, only about 8 proteins could be surface radiodinated on live adult worms. A comparison was also made between the profiles of mael and females worms, isolated from bisexually infected mice. Two major female-specific and one male-specific band were detected by silver and/or Coomassie staining. The female bands, 32 KDa and 18 KDa, partitioned into the detergent and aqueous phase, respectively. The male-specific band of 42 KDa remained in the insoluble phase. Antigenic differences between male and females protins were detected by Western vlotting using a sera from infected Nectomys squamipes.