Differential gene expression studies on HsfA4d, HsfB2a and ClpD1/HSP100 during heat stress in the rice varieties N22 and Co51

In the present investigation, differential expression of two transcription factor genes (HsfA4d and HsfB2a) and a heat shock protein (ClpD1/HSP100) was evaluated in the rice varieties N22 and Co 51 using qRT-PCR. It was observed that gene expression levels were varying during ontogeny. Among the two...

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Main Author:	Rishima Maheendran, V. Soundarrajan, N. M. Manikanda Boopathi, R. Gnanam, V. Geethalakshmi and P. Meenakshisundaram
Format:	Article
Language:	English
Published:	Indian Society of Plant Breeders 2020-12-01
Series:	Electronic Journal of Plant Breeding
Subjects:	rice thermal stress qrt-pcr heat shock factors hsp100 heat stress memory

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spelling	doaj-4d8cf5d272de49019d313fd62f996c7d2021-01-10T17:11:39ZengIndian Society of Plant BreedersElectronic Journal of Plant Breeding0975-928X2020-12-011141165117110.37992/2020.1104.188Differential gene expression studies on HsfA4d, HsfB2a and ClpD1/HSP100 during heat stress in the rice varieties N22 and Co51Rishima Maheendran, V. Soundarrajan, N. M. Manikanda Boopathi, R. Gnanam, V. Geethalakshmi and P. MeenakshisundaramIn the present investigation, differential expression of two transcription factor genes (HsfA4d and HsfB2a) and a heat shock protein (ClpD1/HSP100) was evaluated in the rice varieties N22 and Co 51 using qRT-PCR. It was observed that gene expression levels were varying during ontogeny. Among the two transcription factor genes, HsfB2a which is under the influence of the master regulator HsfA1s was found to have enhanced expression levels than HsfA4d, which acts in a HsfA1s independent pathway. Gene expression level of the heat shock protein ClpD1/HSP100 in the heat tolerant variety N22 was higher than that of the Co 51, implying that Co 51 is relatively less heat tolerant. N22 was observed to have higher heat-shock memory retention than that of Co51. ricethermal stressqrt-pcrheat shock factorshsp100heat stress memory
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Rishima Maheendran, V. Soundarrajan, N. M. Manikanda Boopathi, R. Gnanam, V. Geethalakshmi and P. Meenakshisundaram
spellingShingle	Rishima Maheendran, V. Soundarrajan, N. M. Manikanda Boopathi, R. Gnanam, V. Geethalakshmi and P. Meenakshisundaram Differential gene expression studies on HsfA4d, HsfB2a and ClpD1/HSP100 during heat stress in the rice varieties N22 and Co51 Electronic Journal of Plant Breeding rice thermal stress qrt-pcr heat shock factors hsp100 heat stress memory
author_facet	Rishima Maheendran, V. Soundarrajan, N. M. Manikanda Boopathi, R. Gnanam, V. Geethalakshmi and P. Meenakshisundaram
author_sort	Rishima Maheendran, V. Soundarrajan, N. M. Manikanda Boopathi, R. Gnanam, V. Geethalakshmi and P. Meenakshisundaram
title	Differential gene expression studies on HsfA4d, HsfB2a and ClpD1/HSP100 during heat stress in the rice varieties N22 and Co51
title_short	Differential gene expression studies on HsfA4d, HsfB2a and ClpD1/HSP100 during heat stress in the rice varieties N22 and Co51
title_full	Differential gene expression studies on HsfA4d, HsfB2a and ClpD1/HSP100 during heat stress in the rice varieties N22 and Co51
title_fullStr	Differential gene expression studies on HsfA4d, HsfB2a and ClpD1/HSP100 during heat stress in the rice varieties N22 and Co51
title_full_unstemmed	Differential gene expression studies on HsfA4d, HsfB2a and ClpD1/HSP100 during heat stress in the rice varieties N22 and Co51
title_sort	differential gene expression studies on hsfa4d, hsfb2a and clpd1/hsp100 during heat stress in the rice varieties n22 and co51
publisher	Indian Society of Plant Breeders
series	Electronic Journal of Plant Breeding
issn	0975-928X
publishDate	2020-12-01
description	In the present investigation, differential expression of two transcription factor genes (HsfA4d and HsfB2a) and a heat shock protein (ClpD1/HSP100) was evaluated in the rice varieties N22 and Co 51 using qRT-PCR. It was observed that gene expression levels were varying during ontogeny. Among the two transcription factor genes, HsfB2a which is under the influence of the master regulator HsfA1s was found to have enhanced expression levels than HsfA4d, which acts in a HsfA1s independent pathway. Gene expression level of the heat shock protein ClpD1/HSP100 in the heat tolerant variety N22 was higher than that of the Co 51, implying that Co 51 is relatively less heat tolerant. N22 was observed to have higher heat-shock memory retention than that of Co51.
topic	rice thermal stress qrt-pcr heat shock factors hsp100 heat stress memory
work_keys_str_mv	AT rishimamaheendranvsoundarrajannmmanikandaboopathirgnanamvgeethalakshmiandpmeenakshisundaram differentialgeneexpressionstudiesonhsfa4dhsfb2aandclpd1hsp100duringheatstressinthericevarietiesn22andco51
_version_	1724341752347754496

Differential gene expression studies on HsfA4d, HsfB2a and ClpD1/HSP100 during heat stress in the rice varieties N22 and Co51

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