IN VITRO ANTIPLASMODIAL ACTIVITY OF COUMARIN 8-HYDROXYISOCAPNOLACTONE-2,3-DIOL ISOLATED FROM Micromelum minutum (G. Forst.) Wight & Arn
Malaria remains a health problem in tropical and subtropical countries. The spread of antimalarial resistant parasites has prompted the research to find more effective new antimalarials. One strategy to discover new antimalarial drugs is through the exploration of medicinal plants traditionally...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Gadjah Mada
2014-01-01
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| Series: | Indonesian Journal of Pharmacy |
| Subjects: | |
| Online Access: | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/18 |
| Summary: | Malaria remains a health problem in tropical and subtropical
countries. The spread of antimalarial resistant parasites has
prompted the research to find more effective new antimalarials.
One strategy to discover new antimalarial drugs is through the
exploration of medicinal plants traditionally used to treat malaria.
Micromelum minutum is one of medicinal plants has been used to
treat malaria and other infectious diseases in Malaysia. The
coumarin 8-hydroxyisocapnolactone-2,3-diol has been isolated
from this plant and showed to have strong cytotoxic activities. This
study aimed to evaluate the in vitro antiplasmodial activity of 8-
hydroxyisocapnolactone-2,3-diol against chloroquine-resistant
(FCR-3) and chloroquine-sensitive (D-10) Plasmodium falciparum.
Culture of P. falciparum was continuously grown using a candle jar.
Antiplasmodial assay was conducted by microradioactive method.
The antiplasmodial activity was expressed by the IC50 indicating the
concentration of the compound yielding 50% inhibition of the
parasit growth. The results showed that 8-hydroxyisocapnolactone2,3-diol
has in vitro antiplasmodial activity against FCR-3 and D-10
with the IC50 values of 6.39 µg/mL (16.99 μM) and 24.23 μg/mL
(64.45 μM), respectively. |
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| ISSN: | 2338-9427 2338-9486 |