Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi Arabia
A survey was conducted to determine the status of Lucerne transient streak virus (LTSV) in three high-yielding alfalfa regions in central Saudi Arabia (Riyadh, Qassim, and Hail) during 2014. Three hundred and eight symptomatic alfalfa, and seven Sonchus oleraceus samples were collected. DAS-ELISA in...
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doaj-4d80d689991245f69fb8e949381cad8c2020-11-24T22:32:17ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542017-02-01331435210.5423/PPJ.OA.06.2016.0143PPJ.OA.06.2016.0143Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi ArabiaAhmed Raza0Ibrahim M. Al-Shahwan1Omer A. Abdalla2Mohammed A. Al-Saleh3Mahmoud A. Amer4Department of Plant Protection, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Plant Protection, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Plant Protection, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Plant Protection, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Plant Protection, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaA survey was conducted to determine the status of Lucerne transient streak virus (LTSV) in three high-yielding alfalfa regions in central Saudi Arabia (Riyadh, Qassim, and Hail) during 2014. Three hundred and eight symptomatic alfalfa, and seven Sonchus oleraceus samples were collected. DAS-ELISA indicated that 59 of these samples were positive to LTSV. Two isolates of LTSV from each region were selected for molecular studies. RT-PCR confirmed the presence of LTSV in the selected samples using a specific primer pair. Percentage identity and homology tree comparisons revealed that all Saudi isolates were more closely related to each other but also closely related to the Canadian isolate-JQ782213 (97.1–97.6%) and the New Zealand isolate-U31286 (95.8–97.1%). Comparing Saudi isolates of LTSV with ten other sobemoviruses based on the coat protein gene sequences confirmed the distant relationship between them. Eleven out of fourteen plant species used in host range study were positive to LTSV. This is the first time to document that Trifolium alexandrinum, Nicotiana occidentalis, Chenopodium glaucum, and Lathyrus sativus are new host plant species for LTSV and that N. occidentalis being a good propagative host for it.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291397/alfalfaLucerne transient streak virusSaudi Arabia |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ahmed Raza Ibrahim M. Al-Shahwan Omer A. Abdalla Mohammed A. Al-Saleh Mahmoud A. Amer |
spellingShingle |
Ahmed Raza Ibrahim M. Al-Shahwan Omer A. Abdalla Mohammed A. Al-Saleh Mahmoud A. Amer Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi Arabia The Plant Pathology Journal alfalfa Lucerne transient streak virus Saudi Arabia |
author_facet |
Ahmed Raza Ibrahim M. Al-Shahwan Omer A. Abdalla Mohammed A. Al-Saleh Mahmoud A. Amer |
author_sort |
Ahmed Raza |
title |
Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi Arabia |
title_short |
Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi Arabia |
title_full |
Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi Arabia |
title_fullStr |
Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi Arabia |
title_full_unstemmed |
Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi Arabia |
title_sort |
lucerne transient streak virus; a recently detected virus infecting alfafa (medicago sativa) in central saudi arabia |
publisher |
Hanrimwon Publishing Company |
series |
The Plant Pathology Journal |
issn |
1598-2254 |
publishDate |
2017-02-01 |
description |
A survey was conducted to determine the status of Lucerne transient streak virus (LTSV) in three high-yielding alfalfa regions in central Saudi Arabia (Riyadh, Qassim, and Hail) during 2014. Three hundred and eight symptomatic alfalfa, and seven Sonchus oleraceus samples were collected. DAS-ELISA indicated that 59 of these samples were positive to LTSV. Two isolates of LTSV from each region were selected for molecular studies. RT-PCR confirmed the presence of LTSV in the selected samples using a specific primer pair. Percentage identity and homology tree comparisons revealed that all Saudi isolates were more closely related to each other but also closely related to the Canadian isolate-JQ782213 (97.1–97.6%) and the New Zealand isolate-U31286 (95.8–97.1%). Comparing Saudi isolates of LTSV with ten other sobemoviruses based on the coat protein gene sequences confirmed the distant relationship between them. Eleven out of fourteen plant species used in host range study were positive to LTSV. This is the first time to document that Trifolium alexandrinum, Nicotiana occidentalis, Chenopodium glaucum, and Lathyrus sativus are new host plant species for LTSV and that N. occidentalis being a good propagative host for it. |
topic |
alfalfa Lucerne transient streak virus Saudi Arabia |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291397/ |
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