Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi Arabia

A survey was conducted to determine the status of Lucerne transient streak virus (LTSV) in three high-yielding alfalfa regions in central Saudi Arabia (Riyadh, Qassim, and Hail) during 2014. Three hundred and eight symptomatic alfalfa, and seven Sonchus oleraceus samples were collected. DAS-ELISA in...

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Main Authors: Ahmed Raza, Ibrahim M. Al-Shahwan, Omer A. Abdalla, Mohammed A. Al-Saleh, Mahmoud A. Amer
Format: Article
Language:English
Published: Hanrimwon Publishing Company 2017-02-01
Series:The Plant Pathology Journal
Subjects:
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291397/
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spelling doaj-4d80d689991245f69fb8e949381cad8c2020-11-24T22:32:17ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542017-02-01331435210.5423/PPJ.OA.06.2016.0143PPJ.OA.06.2016.0143Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi ArabiaAhmed Raza0Ibrahim M. Al-Shahwan1Omer A. Abdalla2Mohammed A. Al-Saleh3Mahmoud A. Amer4Department of Plant Protection, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Plant Protection, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Plant Protection, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Plant Protection, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Plant Protection, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi ArabiaA survey was conducted to determine the status of Lucerne transient streak virus (LTSV) in three high-yielding alfalfa regions in central Saudi Arabia (Riyadh, Qassim, and Hail) during 2014. Three hundred and eight symptomatic alfalfa, and seven Sonchus oleraceus samples were collected. DAS-ELISA indicated that 59 of these samples were positive to LTSV. Two isolates of LTSV from each region were selected for molecular studies. RT-PCR confirmed the presence of LTSV in the selected samples using a specific primer pair. Percentage identity and homology tree comparisons revealed that all Saudi isolates were more closely related to each other but also closely related to the Canadian isolate-JQ782213 (97.1–97.6%) and the New Zealand isolate-U31286 (95.8–97.1%). Comparing Saudi isolates of LTSV with ten other sobemoviruses based on the coat protein gene sequences confirmed the distant relationship between them. Eleven out of fourteen plant species used in host range study were positive to LTSV. This is the first time to document that Trifolium alexandrinum, Nicotiana occidentalis, Chenopodium glaucum, and Lathyrus sativus are new host plant species for LTSV and that N. occidentalis being a good propagative host for it.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291397/alfalfaLucerne transient streak virusSaudi Arabia
collection DOAJ
language English
format Article
sources DOAJ
author Ahmed Raza
Ibrahim M. Al-Shahwan
Omer A. Abdalla
Mohammed A. Al-Saleh
Mahmoud A. Amer
spellingShingle Ahmed Raza
Ibrahim M. Al-Shahwan
Omer A. Abdalla
Mohammed A. Al-Saleh
Mahmoud A. Amer
Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi Arabia
The Plant Pathology Journal
alfalfa
Lucerne transient streak virus
Saudi Arabia
author_facet Ahmed Raza
Ibrahim M. Al-Shahwan
Omer A. Abdalla
Mohammed A. Al-Saleh
Mahmoud A. Amer
author_sort Ahmed Raza
title Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi Arabia
title_short Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi Arabia
title_full Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi Arabia
title_fullStr Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi Arabia
title_full_unstemmed Lucerne transient streak virus; a Recently Detected Virus Infecting Alfafa (Medicago sativa) in Central Saudi Arabia
title_sort lucerne transient streak virus; a recently detected virus infecting alfafa (medicago sativa) in central saudi arabia
publisher Hanrimwon Publishing Company
series The Plant Pathology Journal
issn 1598-2254
publishDate 2017-02-01
description A survey was conducted to determine the status of Lucerne transient streak virus (LTSV) in three high-yielding alfalfa regions in central Saudi Arabia (Riyadh, Qassim, and Hail) during 2014. Three hundred and eight symptomatic alfalfa, and seven Sonchus oleraceus samples were collected. DAS-ELISA indicated that 59 of these samples were positive to LTSV. Two isolates of LTSV from each region were selected for molecular studies. RT-PCR confirmed the presence of LTSV in the selected samples using a specific primer pair. Percentage identity and homology tree comparisons revealed that all Saudi isolates were more closely related to each other but also closely related to the Canadian isolate-JQ782213 (97.1–97.6%) and the New Zealand isolate-U31286 (95.8–97.1%). Comparing Saudi isolates of LTSV with ten other sobemoviruses based on the coat protein gene sequences confirmed the distant relationship between them. Eleven out of fourteen plant species used in host range study were positive to LTSV. This is the first time to document that Trifolium alexandrinum, Nicotiana occidentalis, Chenopodium glaucum, and Lathyrus sativus are new host plant species for LTSV and that N. occidentalis being a good propagative host for it.
topic alfalfa
Lucerne transient streak virus
Saudi Arabia
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5291397/
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