Identification MdeSOS1 in Magnolia denudata and its function in response to salt stress

• Main Authors: Pengjie Chang, Zhen Wu, Nannan Song, Sainan Bian, Ninghang Wang, Lingjuan Xuan, Bin Dong, Xiaode Wang, Yamei Shen
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2020-01-01
• Series: Journal of Plant Interactions
• Subjects: magnolia denudata; salt stress; sos1; transgenic plants; na+/h+ antiporter
• Online Access: http://dx.doi.org/10.1080/17429145.2020.1843721
• ISSN: 1742-9145; 1742-9153

In this study, annual Magnolia denudata seedlings were treated with a 200 mmol L−1 NaCl solution. Na+ content in its stems increased by more than about 24 times after 72 h and K+ content in leaves basically maintained in a steady state. MdeSOS1, isolated from M. denudata, comprised a 3453-bp ORF and included 12 transmembrane structures within its N terminal and a hydrophilic tail in its C-terminal. Its protein shared the identity of 68.3% and 62.7% at the peptide level with the homologue PeSOS1 and AtSOS1 respectively. The MdeSOS1 was significantly induced to up-regulation in various tissues exposed to salt stress and improved the salt tolerance of Arabidopsis. Our results also revealed that the MdeSOS1-GFP fusion protein was located on the plasma membrane and MdeSOS1 encoded a salt-inducible plasma membrane Na+/H+ antiporter, which provides a reference to improve the salt tolerance of Magnolia species by transgenic approaches.