Genome-Wide Analysis of Multiple Organellar RNA Editing Factor Family in Poplar Reveals Evolution and Roles in Drought Stress

Genome-Wide Analysis of Multiple Organellar RNA Editing Factor Family in Poplar Reveals Evolution and Roles in Drought Stress

Poplar (Populus) is one of the most important woody plants worldwide. Drought, a primary abiotic stress, seriously affects poplar growth and development. Multiple organellar RNA editing factor (<i>MORF</i>) genes&#8212;pivotal factors in the RNA editosome in <i>Arabidopsis thal...

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Main Authors: Dongli Wang, Sen Meng, Wanlong Su, Yu Bao, Yingying Lu, Weilun Yin, Chao Liu, Xinli Xia
Format: Article
Language:English
Published: MDPI AG 2019-03-01
Series:International Journal of Molecular Sciences
Subjects:
<i>Populus trichocarpa</i>
multiple organellar RNA editing factor
drought stress
RNA editing
genome
Online Access:https://www.mdpi.com/1422-0067/20/6/1425
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spelling doaj-4d45483f01534cc18e09a351126a28bf2020-11-24T21:44:33ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-03-01206142510.3390/ijms20061425ijms20061425Genome-Wide Analysis of Multiple Organellar RNA Editing Factor Family in Poplar Reveals Evolution and Roles in Drought StressDongli Wang0Sen Meng1Wanlong Su2Yu Bao3Yingying Lu4Weilun Yin5Chao Liu6Xinli Xia7Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaBeijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, ChinaPoplar (Populus) is one of the most important woody plants worldwide. Drought, a primary abiotic stress, seriously affects poplar growth and development. Multiple organellar RNA editing factor (<i>MORF</i>) genes&#8212;pivotal factors in the RNA editosome in <i>Arabidopsis thaliana</i>&#8212;are indispensable for the regulation of various physiological processes, including organelle C-to-U RNA editing and plasmid development, as well as in the response to stresses. Although the poplar genome sequence has been released, little is known about <i>MORF</i> genes in poplar, especially those involved in the response to drought stress at the genome-wide level. In this study, we identified nine <i>MORF</i> genes in the Populus genome. Based on the structural features of MORF proteins and the topology of the phylogenetic tree, the <i>P. trichocarpa</i> (Ptr) MORF family members were classified into six groups (Groups I&#8211;VI). A microsynteny analysis indicated that two (22.2%) <i>PtrMORF</i> genes were tandemly duplicated and seven genes (77.8%) were segmentally duplicated. Based on the <i>d<sub>N</sub>/d<sub>S</sub></i> ratios, purifying selection likely played a major role in the evolution of this family and contributed to functional divergence among <i>PtrMORF</i> genes. Moreover, analysis of qRT-PCR data revealed that <i>PtrMORFs</i> exhibited tissue- and treatment-specific expression patterns. <i>PtrMORF</i> genes in all group were involved in the stress response. These results provide a solid foundation for further analyses of the functions and molecular evolution of <i>MORF</i> genes in poplar, and, in particular, for improving the drought resistance of poplar by genetics manipulation.https://www.mdpi.com/1422-0067/20/6/1425<i>Populus trichocarpa</i>multiple organellar RNA editing factordrought stressRNA editinggenome
collection DOAJ
language English
format Article
sources DOAJ
author Dongli Wang
Sen Meng
Wanlong Su
Yu Bao
Yingying Lu
Weilun Yin
Chao Liu
Xinli Xia
spellingShingle Dongli Wang
Sen Meng
Wanlong Su
Yu Bao
Yingying Lu
Weilun Yin
Chao Liu
Xinli Xia
Genome-Wide Analysis of Multiple Organellar RNA Editing Factor Family in Poplar Reveals Evolution and Roles in Drought Stress
International Journal of Molecular Sciences
<i>Populus trichocarpa</i>
multiple organellar RNA editing factor
drought stress
RNA editing
genome
author_facet Dongli Wang
Sen Meng
Wanlong Su
Yu Bao
Yingying Lu
Weilun Yin
Chao Liu
Xinli Xia
author_sort Dongli Wang
title Genome-Wide Analysis of Multiple Organellar RNA Editing Factor Family in Poplar Reveals Evolution and Roles in Drought Stress
title_short Genome-Wide Analysis of Multiple Organellar RNA Editing Factor Family in Poplar Reveals Evolution and Roles in Drought Stress
title_full Genome-Wide Analysis of Multiple Organellar RNA Editing Factor Family in Poplar Reveals Evolution and Roles in Drought Stress
title_fullStr Genome-Wide Analysis of Multiple Organellar RNA Editing Factor Family in Poplar Reveals Evolution and Roles in Drought Stress
title_full_unstemmed Genome-Wide Analysis of Multiple Organellar RNA Editing Factor Family in Poplar Reveals Evolution and Roles in Drought Stress
title_sort genome-wide analysis of multiple organellar rna editing factor family in poplar reveals evolution and roles in drought stress
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1422-0067
publishDate 2019-03-01
description Poplar (Populus) is one of the most important woody plants worldwide. Drought, a primary abiotic stress, seriously affects poplar growth and development. Multiple organellar RNA editing factor (<i>MORF</i>) genes&#8212;pivotal factors in the RNA editosome in <i>Arabidopsis thaliana</i>&#8212;are indispensable for the regulation of various physiological processes, including organelle C-to-U RNA editing and plasmid development, as well as in the response to stresses. Although the poplar genome sequence has been released, little is known about <i>MORF</i> genes in poplar, especially those involved in the response to drought stress at the genome-wide level. In this study, we identified nine <i>MORF</i> genes in the Populus genome. Based on the structural features of MORF proteins and the topology of the phylogenetic tree, the <i>P. trichocarpa</i> (Ptr) MORF family members were classified into six groups (Groups I&#8211;VI). A microsynteny analysis indicated that two (22.2%) <i>PtrMORF</i> genes were tandemly duplicated and seven genes (77.8%) were segmentally duplicated. Based on the <i>d<sub>N</sub>/d<sub>S</sub></i> ratios, purifying selection likely played a major role in the evolution of this family and contributed to functional divergence among <i>PtrMORF</i> genes. Moreover, analysis of qRT-PCR data revealed that <i>PtrMORFs</i> exhibited tissue- and treatment-specific expression patterns. <i>PtrMORF</i> genes in all group were involved in the stress response. These results provide a solid foundation for further analyses of the functions and molecular evolution of <i>MORF</i> genes in poplar, and, in particular, for improving the drought resistance of poplar by genetics manipulation.
topic <i>Populus trichocarpa</i>
multiple organellar RNA editing factor
drought stress
RNA editing
genome
url https://www.mdpi.com/1422-0067/20/6/1425
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