Analysis of QTLs on heading date based on single segment substitution lines in rice (Oryza Sativa L.)

Analysis of QTLs on heading date based on single segment substitution lines in rice (Oryza Sativa L.)

Abstract Single segment substitution lines (SSSLs) have been confirmed to be powerful tools to perform quantitative trait locus (QTL) analysis. This study illuminated the process and methods of QTL analysis with SSSLs on heading date (HD) in rice. QTL identification under two cropping seasons reveal...

Full description

Bibliographic Details
Main Authors: Haitao Zhu, Yun Li, Jiayan Liang, Xin Luan, Pan Xu, Shaokui Wang, Guiquan Zhang, Guifu Liu
Format: Article
Language:English
Published: Nature Publishing Group 2018-09-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-018-31377-7
id doaj-4d3e5594dac3447e856b4bcf994511a3
record_format Article
spelling doaj-4d3e5594dac3447e856b4bcf994511a32020-12-08T06:24:32ZengNature Publishing GroupScientific Reports2045-23222018-09-01811810.1038/s41598-018-31377-7Analysis of QTLs on heading date based on single segment substitution lines in rice (Oryza Sativa L.)Haitao Zhu0Yun Li1Jiayan Liang2Xin Luan3Pan Xu4Shaokui Wang5Guiquan Zhang6Guifu Liu7Guangdong Key Lab of Plant Molecular Breeding, South China Agricultural UniversityGuangdong Key Lab of Plant Molecular Breeding, South China Agricultural UniversityGuangdong Key Lab of Plant Molecular Breeding, South China Agricultural UniversityGuangdong Key Lab of Plant Molecular Breeding, South China Agricultural UniversityCollege of Computational Science, Zhongkai University of Agriculture and EngineeringGuangdong Key Lab of Plant Molecular Breeding, South China Agricultural UniversityGuangdong Key Lab of Plant Molecular Breeding, South China Agricultural UniversityGuangdong Key Lab of Plant Molecular Breeding, South China Agricultural UniversityAbstract Single segment substitution lines (SSSLs) have been confirmed to be powerful tools to perform quantitative trait locus (QTL) analysis. This study illuminated the process and methods of QTL analysis with SSSLs on heading date (HD) in rice. QTL identification under two cropping seasons revealed 98 of 202 SSSLs associated with HD. A total of 22 QTLs were positioned in relative narrow regions on chromosomes by mrMLM.GUI software. QTL qHd3-1 was precisely positioned at 4.4 cM on chromosome 3 by a secondary F2 population. Through SSSL pyramiding, double segment substitution lines were constructed and used to analyze epistatic interactions of digenic loci. Epistatic effects for three pairs of QTLs were estimated, indicating the interactions of QTL qHd3-1 with other QTLs detected and the role to enhance the expression of early ripening or restraining of late flowering QTLs. Additionally, analysis of QTL in different environments provided information about the stability of HD QTLs. This type of research points out the way to excavate favorable genes for design breeding.https://doi.org/10.1038/s41598-018-31377-7
collection DOAJ
language English
format Article
sources DOAJ
author Haitao Zhu
Yun Li
Jiayan Liang
Xin Luan
Pan Xu
Shaokui Wang
Guiquan Zhang
Guifu Liu
spellingShingle Haitao Zhu
Yun Li
Jiayan Liang
Xin Luan
Pan Xu
Shaokui Wang
Guiquan Zhang
Guifu Liu
Analysis of QTLs on heading date based on single segment substitution lines in rice (Oryza Sativa L.)
Scientific Reports
author_facet Haitao Zhu
Yun Li
Jiayan Liang
Xin Luan
Pan Xu
Shaokui Wang
Guiquan Zhang
Guifu Liu
author_sort Haitao Zhu
title Analysis of QTLs on heading date based on single segment substitution lines in rice (Oryza Sativa L.)
title_short Analysis of QTLs on heading date based on single segment substitution lines in rice (Oryza Sativa L.)
title_full Analysis of QTLs on heading date based on single segment substitution lines in rice (Oryza Sativa L.)
title_fullStr Analysis of QTLs on heading date based on single segment substitution lines in rice (Oryza Sativa L.)
title_full_unstemmed Analysis of QTLs on heading date based on single segment substitution lines in rice (Oryza Sativa L.)
title_sort analysis of qtls on heading date based on single segment substitution lines in rice (oryza sativa l.)
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2018-09-01
description Abstract Single segment substitution lines (SSSLs) have been confirmed to be powerful tools to perform quantitative trait locus (QTL) analysis. This study illuminated the process and methods of QTL analysis with SSSLs on heading date (HD) in rice. QTL identification under two cropping seasons revealed 98 of 202 SSSLs associated with HD. A total of 22 QTLs were positioned in relative narrow regions on chromosomes by mrMLM.GUI software. QTL qHd3-1 was precisely positioned at 4.4 cM on chromosome 3 by a secondary F2 population. Through SSSL pyramiding, double segment substitution lines were constructed and used to analyze epistatic interactions of digenic loci. Epistatic effects for three pairs of QTLs were estimated, indicating the interactions of QTL qHd3-1 with other QTLs detected and the role to enhance the expression of early ripening or restraining of late flowering QTLs. Additionally, analysis of QTL in different environments provided information about the stability of HD QTLs. This type of research points out the way to excavate favorable genes for design breeding.
url https://doi.org/10.1038/s41598-018-31377-7
work_keys_str_mv AT haitaozhu analysisofqtlsonheadingdatebasedonsinglesegmentsubstitutionlinesinriceoryzasatival
AT yunli analysisofqtlsonheadingdatebasedonsinglesegmentsubstitutionlinesinriceoryzasatival
AT jiayanliang analysisofqtlsonheadingdatebasedonsinglesegmentsubstitutionlinesinriceoryzasatival
AT xinluan analysisofqtlsonheadingdatebasedonsinglesegmentsubstitutionlinesinriceoryzasatival
AT panxu analysisofqtlsonheadingdatebasedonsinglesegmentsubstitutionlinesinriceoryzasatival
AT shaokuiwang analysisofqtlsonheadingdatebasedonsinglesegmentsubstitutionlinesinriceoryzasatival
AT guiquanzhang analysisofqtlsonheadingdatebasedonsinglesegmentsubstitutionlinesinriceoryzasatival
AT guifuliu analysisofqtlsonheadingdatebasedonsinglesegmentsubstitutionlinesinriceoryzasatival
_version_ 1724391275373789184

Similar Items