<b>Buserelin acetate-induced spermiation in <i>Leptodactylus ocellatus</i> and evaluation of semen characteristics</b> - doi: 10.4025/actascibiolsci.v35i1.14777

• Main Authors: Angela Emi Takamura, Rodrigo Diana Navarro, Allan Reis Troni, Oswaldo Pinto Ribeiro Filho, Jose Cola Zanuncio, Marcelo Maia Pereira
• Format: Article
• Language: English
• Published: Universidade Estadual de Maringá 2012-12-01
• Series: Acta Scientiarum : Biological Sciences
• Subjects: creole frog; GnRHa; semen; spermatozoon; sperm motility
• Online Access: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14777
• ISSN: 1679-9283; 1807-863X

<p class="aresumo">By<strong> </strong>understanding the hormonal action behind the induction of spermiation in <em>Leptodactylus ocellatus </em>can help in the collection of semen for artificial insemination and cryopreservation.<em> </em>The experiments were conducted at the Experimental Frog Farm of the Federal University of<em> </em>Viçosa. Seven <em>L. ocellatus </em>males with the following secondary sexual characteristics were<em> </em>selected: black thorns, developed forelimbs, and amplexus reflex. Spermiation was<em> </em>induced in these animals by applying daily 0.4 μg buserelin acetate hormone (GnRHa) for 7 days, being the semen collected 90 min after the application. The volume, color,<em> </em>vigor, motility, sperm concentration, and spermatozoa morphology were subsequently<em> </em>evaluated. Three animals responded to GnRHa induction, enabling the collection of seven<em> </em>samples of semen, and the first sample was collected 97.5h after the first application. The<em> </em>semen of the creole frog presented the following characteristics: an average volume of 0.38 mL,<em> </em>murky color, sperm vigor of 3.71, sperm motility of 77.14%, sperm concentration of 6.60 x 10⁶ SPTZ mL^-1, and 69% of normal sperm. GnRHa can induce spermiation in the creole frog. Although the volume of collected semen was low, the<em> </em>color, vigor, motility, concentration, and spermatozoa content showed to be adequate.</p> <p class="apalavrachave"> </p>