Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria.

Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria.

Most plasmids replicate only within a particular genus or family.Here we describe an engineered high copy number expression vector, pBAV1K-T5, that produces varying quantities of active reporter proteins in Escherichia coli, Acinetobacter baylyi ADP1, Agrobacterium tumefaciens, (all gram-negative),...

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Bibliographic Details
Main Authors: Anton V Bryksin, Ichiro Matsumura
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-10-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2951906?pdf=render
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spelling doaj-4d198004a4d5446f91312b5adc1a2c5f2020-11-24T20:50:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-10-01510e1324410.1371/journal.pone.0013244Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria.Anton V BryksinIchiro MatsumuraMost plasmids replicate only within a particular genus or family.Here we describe an engineered high copy number expression vector, pBAV1K-T5, that produces varying quantities of active reporter proteins in Escherichia coli, Acinetobacter baylyi ADP1, Agrobacterium tumefaciens, (all gram-negative), Streptococcus pneumoniae, Leifsonia shinshuensis, Peanibacillus sp. S18-36 and Bacillus subtilis (gram-positive).Our results demonstrate the efficiency of pBAV1K-T5 replication in different bacterial species, thereby facilitating the study of proteins that don't fold well in E. coli and pathogens not amenable to existing genetic tools.http://europepmc.org/articles/PMC2951906?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Anton V Bryksin
Ichiro Matsumura
spellingShingle Anton V Bryksin
Ichiro Matsumura
Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria.
PLoS ONE
author_facet Anton V Bryksin
Ichiro Matsumura
author_sort Anton V Bryksin
title Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria.
title_short Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria.
title_full Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria.
title_fullStr Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria.
title_full_unstemmed Rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria.
title_sort rational design of a plasmid origin that replicates efficiently in both gram-positive and gram-negative bacteria.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2010-10-01
description Most plasmids replicate only within a particular genus or family.Here we describe an engineered high copy number expression vector, pBAV1K-T5, that produces varying quantities of active reporter proteins in Escherichia coli, Acinetobacter baylyi ADP1, Agrobacterium tumefaciens, (all gram-negative), Streptococcus pneumoniae, Leifsonia shinshuensis, Peanibacillus sp. S18-36 and Bacillus subtilis (gram-positive).Our results demonstrate the efficiency of pBAV1K-T5 replication in different bacterial species, thereby facilitating the study of proteins that don't fold well in E. coli and pathogens not amenable to existing genetic tools.
url http://europepmc.org/articles/PMC2951906?pdf=render
work_keys_str_mv AT antonvbryksin rationaldesignofaplasmidoriginthatreplicatesefficientlyinbothgrampositiveandgramnegativebacteria
AT ichiromatsumura rationaldesignofaplasmidoriginthatreplicatesefficientlyinbothgrampositiveandgramnegativebacteria
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