Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA

Abstract The increasing risk from viral outbreaks such as the ongoing COVID-19 pandemic exacerbates the need for rapid, affordable and sensitive methods for virus detection, identification and quantification; however, existing methods for detecting virus particles in biological samples usually depen...

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Main Authors:	Christof Hepp, Nicolas Shiaelis, Nicole C. Robb, Alison Vaughan, Philippa C. Matthews, Nicole Stoesser, Derrick Crook, Achillefs N. Kapanidis
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-10-01
Series:	Scientific Reports
Online Access:	https://doi.org/10.1038/s41598-021-98972-z

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spelling	doaj-4d11296b02a54aa2b9477fd8652b90372021-10-03T11:31:01ZengNature Publishing GroupScientific Reports2045-23222021-10-0111111210.1038/s41598-021-98972-zViral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNAChristof Hepp0Nicolas Shiaelis1Nicole C. Robb2Alison Vaughan3Philippa C. Matthews4Nicole Stoesser5Derrick Crook6Achillefs N. Kapanidis7Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of OxfordBiological Physics Research Group, Clarendon Laboratory, Department of Physics, University of OxfordBiological Physics Research Group, Clarendon Laboratory, Department of Physics, University of OxfordNuffield Department for Medicine, University of OxfordNuffield Department for Medicine, University of OxfordNuffield Department for Medicine, University of OxfordNuffield Department for Medicine, University of OxfordBiological Physics Research Group, Clarendon Laboratory, Department of Physics, University of OxfordAbstract The increasing risk from viral outbreaks such as the ongoing COVID-19 pandemic exacerbates the need for rapid, affordable and sensitive methods for virus detection, identification and quantification; however, existing methods for detecting virus particles in biological samples usually depend on multistep protocols that take considerable time to yield a result. Here, we introduce a rapid fluorescence in situ hybridization (FISH) protocol capable of detecting influenza virus, avian infectious bronchitis virus and SARS-CoV-2 specifically and quantitatively in approximately 20 min, in virus cultures, combined nasal and throat swabs with added virus and likely patient samples without previous purification. This fast and facile workflow can be adapted both as a lab technique and a future diagnostic tool in enveloped viruses with an accessible genome.https://doi.org/10.1038/s41598-021-98972-z
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Christof Hepp Nicolas Shiaelis Nicole C. Robb Alison Vaughan Philippa C. Matthews Nicole Stoesser Derrick Crook Achillefs N. Kapanidis
spellingShingle	Christof Hepp Nicolas Shiaelis Nicole C. Robb Alison Vaughan Philippa C. Matthews Nicole Stoesser Derrick Crook Achillefs N. Kapanidis Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA Scientific Reports
author_facet	Christof Hepp Nicolas Shiaelis Nicole C. Robb Alison Vaughan Philippa C. Matthews Nicole Stoesser Derrick Crook Achillefs N. Kapanidis
author_sort	Christof Hepp
title	Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA
title_short	Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA
title_full	Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA
title_fullStr	Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA
title_full_unstemmed	Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA
title_sort	viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral rna
publisher	Nature Publishing Group
series	Scientific Reports
issn	2045-2322
publishDate	2021-10-01
description	Abstract The increasing risk from viral outbreaks such as the ongoing COVID-19 pandemic exacerbates the need for rapid, affordable and sensitive methods for virus detection, identification and quantification; however, existing methods for detecting virus particles in biological samples usually depend on multistep protocols that take considerable time to yield a result. Here, we introduce a rapid fluorescence in situ hybridization (FISH) protocol capable of detecting influenza virus, avian infectious bronchitis virus and SARS-CoV-2 specifically and quantitatively in approximately 20 min, in virus cultures, combined nasal and throat swabs with added virus and likely patient samples without previous purification. This fast and facile workflow can be adapted both as a lab technique and a future diagnostic tool in enveloped viruses with an accessible genome.
url	https://doi.org/10.1038/s41598-021-98972-z
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Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA

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