Headspace Gas Chromatography-Mass Spectrometry for Volatile Components Analysis in <i>Ipomoea Cairica</i> (L.) <i>Sweet</i> Leaves: Natural Deep Eutectic Solvents as Green Extraction and Dilution Matrix

• Main Authors: Wei Zhang, Xianrui Liang
• Format: Article
• Language: English
• Published: MDPI AG 2019-06-01
• Series: Foods
• Subjects: <i>Ipomoea cairica</i> (L.) <i>Sweet</i> leaves; natural deep eutectic solvents; static headspace gas chromatography-mass spectrometry; volatile components
• Online Access: https://www.mdpi.com/2304-8158/8/6/205

In this study, natural deep eutectic solvents (NADESs) were used as both the extraction and dilution matrix in static headspace gas chromatography-mass spectrometry (SHS-GC-MS) for the analysis of volatile components in <i>Ipomoea cairica</i> (L). <i>Sweet</i> (<i>ICS</i>) leaves. Six NADESs were prepared and the NADESs composed of choline chloride and glucose with a 1:1 molar ratio containing 15% water were preferred due to the better peak responses. A total of 77 volatiles in <i>ICS</i> leaves were detected and tentatively identified by mass spectral matching with the US National Institute of Standards and Technology (NIST, 2014) Mass Spectral Library and the retention index-assisted qualitative method. These 77 volatile components were mainly terpenoids, aromatics, and aliphatics. Among them, &#946;-elemene, &#946;-caryophyllene, &#945;-humulene, and 2, 4-di-tert-butylphenol were found to be the main components. This investigation verified that the use of NADESs is an efficient green extraction and dilution matrix of the SHS-GC-MS method for direct volatile component analysis of plant materials without extra extraction work.