The in vitro and in vivo biological effects and osteogenic activity of novel biodegradable porous Mg alloy scaffolds
Inspired by the process of bone reconstruction, porous scaffolds with robust osteogenesis and biodegradability would provide an ideal bone substitute for clinical practice. In this study, a novel porous Mg–Nd–Zn–Zr (JDBM) alloy scaffold coated with brushite (i.e., DCPD), denoted as JDBM-DCPD, is fab...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-04-01
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| Series: | Materials & Design |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127520300472 |
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doaj-4d017a1a94b042e9ad3cb86d526ea8c7 |
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Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Wei Wang Gaozhi Jia Qing Wang Hua Huang Xiaolin Li Hui Zeng Wenjiang Ding Frank Witte Changqing Zhang Weitao Jia Guangyin Yuan |
| spellingShingle |
Wei Wang Gaozhi Jia Qing Wang Hua Huang Xiaolin Li Hui Zeng Wenjiang Ding Frank Witte Changqing Zhang Weitao Jia Guangyin Yuan The in vitro and in vivo biological effects and osteogenic activity of novel biodegradable porous Mg alloy scaffolds Materials & Design |
| author_facet |
Wei Wang Gaozhi Jia Qing Wang Hua Huang Xiaolin Li Hui Zeng Wenjiang Ding Frank Witte Changqing Zhang Weitao Jia Guangyin Yuan |
| author_sort |
Wei Wang |
| title |
The in vitro and in vivo biological effects and osteogenic activity of novel biodegradable porous Mg alloy scaffolds |
| title_short |
The in vitro and in vivo biological effects and osteogenic activity of novel biodegradable porous Mg alloy scaffolds |
| title_full |
The in vitro and in vivo biological effects and osteogenic activity of novel biodegradable porous Mg alloy scaffolds |
| title_fullStr |
The in vitro and in vivo biological effects and osteogenic activity of novel biodegradable porous Mg alloy scaffolds |
| title_full_unstemmed |
The in vitro and in vivo biological effects and osteogenic activity of novel biodegradable porous Mg alloy scaffolds |
| title_sort |
in vitro and in vivo biological effects and osteogenic activity of novel biodegradable porous mg alloy scaffolds |
| publisher |
Elsevier |
| series |
Materials & Design |
| issn |
0264-1275 |
| publishDate |
2020-04-01 |
| description |
Inspired by the process of bone reconstruction, porous scaffolds with robust osteogenesis and biodegradability would provide an ideal bone substitute for clinical practice. In this study, a novel porous Mg–Nd–Zn–Zr (JDBM) alloy scaffold coated with brushite (i.e., DCPD), denoted as JDBM-DCPD, is fabricated using a special patent template technique, which forms the main spherical pores (400–450 μm) and smaller pores (150–250 μm) interconnected between the adjacent main pores, facilitating nutrient penetration and cell in-growth, exhibiting sufficient mechanical properties. In vitro results demonstrate that JDBM-DCPD scaffolds promote cell in-growth and osteogenic differentiation, which significantly enhance mineralization, osteogenesis and angiogenesis-related genes expression when cultured with bone marrow mesenchymal stem cells. After implanting in vivo, JDBM-DCPD scaffolds effectively stimulate angiogenesis, osteogenesis, and remodeling with the degradation of JDBM-DCPD, and perfectly repair large bone defect in rat and rabbit models. Results from the present study provide the solid evidence that porous biodegradable Mg-based scaffolds whose pore structure can be precisely regulated by a spacer-selection technique may be a promising tissue engineering scaffolds for large bone defect repair clinically, free from any extra growth factors and live cells, such advances will make scaffold-based bone tissue repair safer, more convenient and more cost-effective. Keywords: Biodegradable Mg–Nd–Zn–Zr alloy scaffold, DCPD coating, Osteogenesis, Angiogenesis, Bone tissue engineering |
| url |
http://www.sciencedirect.com/science/article/pii/S0264127520300472 |
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doaj-4d017a1a94b042e9ad3cb86d526ea8c72020-11-25T00:42:12ZengElsevierMaterials & Design0264-12752020-04-01189The in vitro and in vivo biological effects and osteogenic activity of novel biodegradable porous Mg alloy scaffoldsWei Wang0Gaozhi Jia1Qing Wang2Hua Huang3Xiaolin Li4Hui Zeng5Wenjiang Ding6Frank Witte7Changqing Zhang8Weitao Jia9Guangyin Yuan10Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, ChinaNational Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China; National & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Department of Bone & Joint Surgery, Peking University Shenzhen Hospital, Shenzhen 518036, ChinaDepartment of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, ChinaNational Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Innovation Institute for Materials, Shanghai 200444, ChinaDepartment of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, ChinaNational & Local Joint Engineering Research Center of Orthopaedic Biomaterials, Department of Bone & Joint Surgery, Peking University Shenzhen Hospital, Shenzhen 518036, ChinaNational Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Innovation Institute for Materials, Shanghai 200444, ChinaDepartment of Prostodontics, Charité-Universitätsmedizin Berlin, Assmannshauser Strasse 4-6, Berlin 14197, GermanyDepartment of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China; Corresponding authors.Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, China; Corresponding authors.National Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Innovation Institute for Materials, Shanghai 200444, China; Correspondence to: G. Yuan, National Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Composite, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.Inspired by the process of bone reconstruction, porous scaffolds with robust osteogenesis and biodegradability would provide an ideal bone substitute for clinical practice. In this study, a novel porous Mg–Nd–Zn–Zr (JDBM) alloy scaffold coated with brushite (i.e., DCPD), denoted as JDBM-DCPD, is fabricated using a special patent template technique, which forms the main spherical pores (400–450 μm) and smaller pores (150–250 μm) interconnected between the adjacent main pores, facilitating nutrient penetration and cell in-growth, exhibiting sufficient mechanical properties. In vitro results demonstrate that JDBM-DCPD scaffolds promote cell in-growth and osteogenic differentiation, which significantly enhance mineralization, osteogenesis and angiogenesis-related genes expression when cultured with bone marrow mesenchymal stem cells. After implanting in vivo, JDBM-DCPD scaffolds effectively stimulate angiogenesis, osteogenesis, and remodeling with the degradation of JDBM-DCPD, and perfectly repair large bone defect in rat and rabbit models. Results from the present study provide the solid evidence that porous biodegradable Mg-based scaffolds whose pore structure can be precisely regulated by a spacer-selection technique may be a promising tissue engineering scaffolds for large bone defect repair clinically, free from any extra growth factors and live cells, such advances will make scaffold-based bone tissue repair safer, more convenient and more cost-effective. Keywords: Biodegradable Mg–Nd–Zn–Zr alloy scaffold, DCPD coating, Osteogenesis, Angiogenesis, Bone tissue engineeringhttp://www.sciencedirect.com/science/article/pii/S0264127520300472 |