Molecular Detection of Hemoplasma Species (Mycoplasma spp.) in Cattle in Cebu, Philippines

Epidemiological reports on vector-borne diseases affecting cattle in the Philippines have been limited. The present study aimed to detect the presence of the Hemoplasma pathogen (Mycoplasma spp.) in dispersal cattle in selected plainlands and marginal uplands in Cebu, Philippines, using peripheral b...

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Main Authors: Adrian Patalinghug Ybañez, Rochelle Haidee DaclanYbañez, Michihito Tagawa
Format: Article
Language:English
Published: Assiut University 2015-01-01
Series:Journal of Advanced Veterinary Research
Online Access:http://advetresearch.com/index.php/AVR/article/view/45
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spelling doaj-4ce77f49435c465e907382b5398f68702020-11-24T22:15:21ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772015-01-0151434645Molecular Detection of Hemoplasma Species (Mycoplasma spp.) in Cattle in Cebu, PhilippinesAdrian Patalinghug YbañezRochelle Haidee DaclanYbañezMichihito TagawaEpidemiological reports on vector-borne diseases affecting cattle in the Philippines have been limited. The present study aimed to detect the presence of the Hemoplasma pathogen (Mycoplasma spp.) in dispersal cattle in selected plainlands and marginal uplands in Cebu, Philippines, using peripheral blood smear examination (PBSE) and polymerase chain reaction (PCR) methods. A total of 14 cattle were tested. Using PBSE, no inclusion bodies were observed. However, using PCR, 4 out of 14 cattle showed positive results. Results indicate the high sensitivity of the PCR methods in detecting Mycoplasma spp. than that of the PBSE. The present study adds new information on the biodiversity of vector-borne pathogens in cattle in Cebu, Philippines, and is the first report of detection in the country.http://advetresearch.com/index.php/AVR/article/view/45
collection DOAJ
language English
format Article
sources DOAJ
author Adrian Patalinghug Ybañez
Rochelle Haidee DaclanYbañez
Michihito Tagawa
spellingShingle Adrian Patalinghug Ybañez
Rochelle Haidee DaclanYbañez
Michihito Tagawa
Molecular Detection of Hemoplasma Species (Mycoplasma spp.) in Cattle in Cebu, Philippines
Journal of Advanced Veterinary Research
author_facet Adrian Patalinghug Ybañez
Rochelle Haidee DaclanYbañez
Michihito Tagawa
author_sort Adrian Patalinghug Ybañez
title Molecular Detection of Hemoplasma Species (Mycoplasma spp.) in Cattle in Cebu, Philippines
title_short Molecular Detection of Hemoplasma Species (Mycoplasma spp.) in Cattle in Cebu, Philippines
title_full Molecular Detection of Hemoplasma Species (Mycoplasma spp.) in Cattle in Cebu, Philippines
title_fullStr Molecular Detection of Hemoplasma Species (Mycoplasma spp.) in Cattle in Cebu, Philippines
title_full_unstemmed Molecular Detection of Hemoplasma Species (Mycoplasma spp.) in Cattle in Cebu, Philippines
title_sort molecular detection of hemoplasma species (mycoplasma spp.) in cattle in cebu, philippines
publisher Assiut University
series Journal of Advanced Veterinary Research
issn 2090-6269
2090-6277
publishDate 2015-01-01
description Epidemiological reports on vector-borne diseases affecting cattle in the Philippines have been limited. The present study aimed to detect the presence of the Hemoplasma pathogen (Mycoplasma spp.) in dispersal cattle in selected plainlands and marginal uplands in Cebu, Philippines, using peripheral blood smear examination (PBSE) and polymerase chain reaction (PCR) methods. A total of 14 cattle were tested. Using PBSE, no inclusion bodies were observed. However, using PCR, 4 out of 14 cattle showed positive results. Results indicate the high sensitivity of the PCR methods in detecting Mycoplasma spp. than that of the PBSE. The present study adds new information on the biodiversity of vector-borne pathogens in cattle in Cebu, Philippines, and is the first report of detection in the country.
url http://advetresearch.com/index.php/AVR/article/view/45
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