Identificação de loci microssatélitescom potencial de amplificação na espécie de Peixe-Rei (Odontesthes humensis)

Identificação de loci microssatélitescom potencial de amplificação na espécie de Peixe-Rei (Odontesthes humensis)

In this work, 25,806 potentially amplifiable microsatellite loci (PAL) were identified in pejerrey, (Odontesthes humensis), with 21% of dinucleotide, 22% trinucleotide, 37% tetranucleotide, 13% pentanucleotide and 7% hexanucleotide. Of the total loci, 167 were classified as "Best PAL", mor...

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Bibliographic Details
Main Authors: R.A. Tavares, S.R.N. Piedras, M.D. Nunes, D.B. Almeida, C.G.A. Moreira, J.M. Fernandes, S.F. Freitas, H.L.M. Moreira, J.L.O.F. Pouey, N.J.L. Dionello
Format: Article
Language:English
Published: Universidade Federal de Minas Gerais 2014-12-01
Series:Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Subjects:
peixe-rei
microssatélites
marcadores moleculares
PAL
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601941&lng=en&tlng=en
Description
Summary:In this work, 25,806 potentially amplifiable microsatellite loci (PAL) were identified in pejerrey, (Odontesthes humensis), with 21% of dinucleotide, 22% trinucleotide, 37% tetranucleotide, 13% pentanucleotide and 7% hexanucleotide. Of the total loci, 167 were classified as "Best PAL", more likely to be variables in populations. The results show that with a small coverage of the genome it was possible to identify a large number of microsatellite loci.
ISSN:1678-4162

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