Isolation and Characterization of Human Umbilical Cord Wharton’s jelly Stem Cells

• Main Authors: Saber Zahri, Masoud Maleki, Kamaladdin Hamidi, Seiyeh Mahsa Khatami
• Format: Article
• Language: fas
• Published: Ardabil University of Medical Sciences 2013-04-01
• Series: Journal of Ardabil University of Medical Sciences
• Subjects: Umbilical Cord; Mesenchymal Stem Cells; CD105; CD44; CD34
• Online Access: http://jarums.arums.ac.ir/browse.php?a_code=A-10-27-100&slc_lang=en&sid=1
• ISSN: 2228-7280; 2228-7299

Background & Objectives: Stem cells are fundamental supporter of multicellular tissue. They allow blood, bone, gametes, epithelia, nervous system, muscle, and other tissues to be replaced by fresh cells throughout life. In recent years human Wharton’s jelly stem cells (WJSCs) have gained attention. They express a number of surface markers characteristic of mesenchymal stem cells. In this study, human Wharton’s jelly stem cells were isolated using explant method. To show the stemness property of these cells, three CD markers including CD105, CD44 and CD34 were tested.   Methods: The umbilical cord samples were collected by Caesarian section at Arta Hospital in Ardabil. Cords were transferred in sterile conditions and stem cells were isolated using explant method. After log phase, cells were passaged then growth characteristics and CD105, CD44 and CD34 markers investigated by RT-PCR.   Results: Separation of human Wharton’s jelly stem cells were started after 7 days. WJSCs in culture revealed two distinct cell population named Type 1 and Type 2. RT-PCR results showed that WJSCs were CD105+, CD44+ and CD34-.   Conclusion: Human umbilical cord stem cells could be an alternative source instead bone marrow stem cells for cell therapy and tissue engineering. These cells have a fibroblastic appearance. Following the lag phase and into log phase respectively, cells grow easily in culture and retain stemness properties in higher passages.