Development and Optimization of a Fluorescent Imaging System to Detect Amyloid-β Proteins: Phantom Study

Alzheimer disease is the most common form of dementia, affecting more than 5 million people in the United States. During the progression of Alzheimer disease, a particular protein begins to accumulate in the brain and also in extensions of the brain, ie, the retina. This protein, amyloid-β (Aβ), exh...

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Main Authors:	David Tes, Karl Kratkiewicz, Ahmed Aber, Luke Horton, Mohsin Zafar, Nour Arafat, Afreen Fatima, Mohammad RN Avanaki
Format:	Article
Language:	English
Published:	SAGE Publishing 2018-06-01
Series:	Biomedical Engineering and Computational Biology
Online Access:	https://doi.org/10.1177/1179597218781081

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spelling	doaj-4cc3d03906d04e20b154a4560d6445ca2020-11-25T03:16:58ZengSAGE PublishingBiomedical Engineering and Computational Biology1179-59722018-06-01910.1177/1179597218781081Development and Optimization of a Fluorescent Imaging System to Detect Amyloid-β Proteins: Phantom StudyDavid Tes0Karl Kratkiewicz1Ahmed Aber2Luke Horton3Mohsin Zafar4Nour Arafat5Afreen Fatima6Mohammad RN Avanaki7Department of Biomedical Engineering, College of Engineering, Wayne State University, Detroit, MI, USADepartment of Biomedical Engineering, College of Engineering, Wayne State University, Detroit, MI, USASchool of Health and Related Research, University of Sheffield, Sheffield, UKDepartment of Dermatology, Wayne State University, Detroit, MI, USADepartment of Biomedical Engineering, College of Engineering, Wayne State University, Detroit, MI, USADepartment of Biomedical Engineering, College of Engineering, Wayne State University, Detroit, MI, USADepartment of Biomedical Engineering, College of Engineering, Wayne State University, Detroit, MI, USADepartment of Dermatology, Wayne State University, Detroit, MI, USAAlzheimer disease is the most common form of dementia, affecting more than 5 million people in the United States. During the progression of Alzheimer disease, a particular protein begins to accumulate in the brain and also in extensions of the brain, ie, the retina. This protein, amyloid-β (Aβ), exhibits fluorescent properties. The purpose of this research article is to explore the implications of designing a fluorescent imaging system able to detect Aβ proteins in the retina. We designed and implemented a fluorescent imaging system with a range of applications that can be reconfigured on a fluorophore to fluorophore basis and tested its feasibility and capabilities using Cy5 and CRANAD-2 imaging probes. The results indicate a promising potential for the imaging system to be used to study the Aβ biomarker. A performance evaluation involving ex vivo and in vivo experiments is planned for future study.https://doi.org/10.1177/1179597218781081
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	David Tes Karl Kratkiewicz Ahmed Aber Luke Horton Mohsin Zafar Nour Arafat Afreen Fatima Mohammad RN Avanaki
spellingShingle	David Tes Karl Kratkiewicz Ahmed Aber Luke Horton Mohsin Zafar Nour Arafat Afreen Fatima Mohammad RN Avanaki Development and Optimization of a Fluorescent Imaging System to Detect Amyloid-β Proteins: Phantom Study Biomedical Engineering and Computational Biology
author_facet	David Tes Karl Kratkiewicz Ahmed Aber Luke Horton Mohsin Zafar Nour Arafat Afreen Fatima Mohammad RN Avanaki
author_sort	David Tes
title	Development and Optimization of a Fluorescent Imaging System to Detect Amyloid-β Proteins: Phantom Study
title_short	Development and Optimization of a Fluorescent Imaging System to Detect Amyloid-β Proteins: Phantom Study
title_full	Development and Optimization of a Fluorescent Imaging System to Detect Amyloid-β Proteins: Phantom Study
title_fullStr	Development and Optimization of a Fluorescent Imaging System to Detect Amyloid-β Proteins: Phantom Study
title_full_unstemmed	Development and Optimization of a Fluorescent Imaging System to Detect Amyloid-β Proteins: Phantom Study
title_sort	development and optimization of a fluorescent imaging system to detect amyloid-β proteins: phantom study
publisher	SAGE Publishing
series	Biomedical Engineering and Computational Biology
issn	1179-5972
publishDate	2018-06-01
description	Alzheimer disease is the most common form of dementia, affecting more than 5 million people in the United States. During the progression of Alzheimer disease, a particular protein begins to accumulate in the brain and also in extensions of the brain, ie, the retina. This protein, amyloid-β (Aβ), exhibits fluorescent properties. The purpose of this research article is to explore the implications of designing a fluorescent imaging system able to detect Aβ proteins in the retina. We designed and implemented a fluorescent imaging system with a range of applications that can be reconfigured on a fluorophore to fluorophore basis and tested its feasibility and capabilities using Cy5 and CRANAD-2 imaging probes. The results indicate a promising potential for the imaging system to be used to study the Aβ biomarker. A performance evaluation involving ex vivo and in vivo experiments is planned for future study.
url	https://doi.org/10.1177/1179597218781081
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Development and Optimization of a Fluorescent Imaging System to Detect Amyloid-β Proteins: Phantom Study

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