Optimisation of the Method of Stem Cells Mediated Tissue Remodelling in Diabetic Wound Healing
Introduction: Incomplete, slow or limited wound healing is one of the major disabling events accompanying diabetic patients. At the present time, there is no effective treatments which enable efficient wound healing in diabetic patients. Aim: To optimise the method of loading of Human Mesenchyma...
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2020-11-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/14245/46692_CE[Ra1]_F(SL)_PF1(AG_KM)_PFA(KM)_PB(AG_KM)_PN(SL).pdf |
| Summary: | Introduction: Incomplete, slow or limited wound healing is one
of the major disabling events accompanying diabetic patients.
At the present time, there is no effective treatments which
enable efficient wound healing in diabetic patients.
Aim: To optimise the method of loading of Human Mesenchymal
Stem Cells (MSC) with S-Allyl Cysteine (SAC) from aged
garlic extract and invitro optimisation of the delivery process,
attachment and optimisation of stem cells and loaded stem cells
to the fibroin matrix in induction of wound recovery completion
in a model of diabetic wound healing.
Materials and Methods: It was a preclinical invitro model which
employed the anti-inflammatory and anti-bacterial properties of
fibroin matrix after populating them with drug-loaded human
MSCs. This combination allowed slow and sustained release
of the active substance into the wound and with the stem cells,
promoted tissue remodelling thereby, inducing enhanced wound
recovery in diabetic model.
Results: Mixture of SAC and N-Acetylcysteine (NAC) was
most effective. S-Allyl Mercaptocysteine (SAMC) protected
angiogenesis and showed tube formation in the presence of
advanced glycation end-products. Protection against oxidative
stress and apoptosis-Propidium Iodide (PI) staining shows
protective effects of the cysteine compounds particularly SAC
and SAMC.
Conclusion: Present study results provide possibilities of
utilising tissue-engineered fibroin matrix with drug loaded human
MSC as a potential therapeutic technique, due to its beneficial
effects in terms of cell attachment and bioactive factor loading,
for diabetic wound healing. |
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| ISSN: | 2249-782X 0973-709X |