ONAC066, A Stress-Responsive NAC Transcription Activator, Positively Contributes to Rice Immunity Against Magnaprothe oryzae Through Modulating Expression of OsWRKY62 and Three Cytochrome P450 Genes

ONAC066, A Stress-Responsive NAC Transcription Activator, Positively Contributes to Rice Immunity Against Magnaprothe oryzae Through Modulating Expression of OsWRKY62 and Three Cytochrome P450 Genes

NAC transcriptional factors constitute a large family in rice and some of them have been demonstrated to play crucial roles in rice immunity. The present study investigated the function and mechanism of ONAC066 in rice immunity. ONAC066 shows transcription activator activity that depends on its C-te...

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Main Authors: Xi Yuan, Hui Wang, Yan Bi, Yuqing Yan, Yizhou Gao, Xiaohui Xiong, Jiajing Wang, Dayong Li, Fengming Song
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-09-01
Series:Frontiers in Plant Science
Subjects:
NAC (NAM, ATAF, and CUC)
ONAC066
rice immunity
OsWRKY62
cytochrome P450s
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2021.749186/full
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spelling doaj-4cbaa78b29714254bf89994eddb255a12021-09-09T09:48:49ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-09-011210.3389/fpls.2021.749186749186ONAC066, A Stress-Responsive NAC Transcription Activator, Positively Contributes to Rice Immunity Against Magnaprothe oryzae Through Modulating Expression of OsWRKY62 and Three Cytochrome P450 GenesXi Yuan0Xi Yuan1Hui Wang2Yan Bi3Yuqing Yan4Yizhou Gao5Xiaohui Xiong6Jiajing Wang7Dayong Li8Fengming Song9National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaCollege of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, ChinaNational Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaNational Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaNational Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaNational Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaNational Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaNational Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaNational Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaNational Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaNAC transcriptional factors constitute a large family in rice and some of them have been demonstrated to play crucial roles in rice immunity. The present study investigated the function and mechanism of ONAC066 in rice immunity. ONAC066 shows transcription activator activity that depends on its C-terminal region in rice cells. ONAC066-OE plants exhibited enhanced resistance while ONAC066-Ri and onac066-1 plants showed attenuated resistance to Magnaporthe oryzae. A total of 81 genes were found to be up-regulated in ONAC066-OE plants, and 26 of them were predicted to be induced by M. oryzae. Four OsWRKY genes, including OsWRKY45 and OsWRKY62, were up-regulated in ONAC066-OE plants but down-regulated in ONAC066-Ri plants. ONAC066 bound to NAC core-binding site in OsWRKY62 promoter and activated OsWRKY62 expression, indicating that OsWRKY62 is a ONAC066 target. A set of cytochrome P450 genes were found to be co-expressed with ONAC066 and 5 of them were up-regulated in ONAC066-OE plants but down-regulated in ONAC066-Ri plants. ONAC066 bound to promoters of cytochrome P450 genes LOC_Os02g30110, LOC_Os06g37300, and LOC_Os02g36150 and activated their transcription, indicating that these three cytochrome P450 genes are ONAC066 targets. These results suggest that ONAC066, as a transcription activator, positively contributes to rice immunity through modulating the expression of OsWRKY62 and a set of cytochrome P450 genes to activate defense response.https://www.frontiersin.org/articles/10.3389/fpls.2021.749186/fullNAC (NAM, ATAF, and CUC)ONAC066rice immunityOsWRKY62cytochrome P450s
collection DOAJ
language English
format Article
sources DOAJ
author Xi Yuan
Xi Yuan
Hui Wang
Yan Bi
Yuqing Yan
Yizhou Gao
Xiaohui Xiong
Jiajing Wang
Dayong Li
Fengming Song
spellingShingle Xi Yuan
Xi Yuan
Hui Wang
Yan Bi
Yuqing Yan
Yizhou Gao
Xiaohui Xiong
Jiajing Wang
Dayong Li
Fengming Song
ONAC066, A Stress-Responsive NAC Transcription Activator, Positively Contributes to Rice Immunity Against Magnaprothe oryzae Through Modulating Expression of OsWRKY62 and Three Cytochrome P450 Genes
Frontiers in Plant Science
NAC (NAM, ATAF, and CUC)
ONAC066
rice immunity
OsWRKY62
cytochrome P450s
author_facet Xi Yuan
Xi Yuan
Hui Wang
Yan Bi
Yuqing Yan
Yizhou Gao
Xiaohui Xiong
Jiajing Wang
Dayong Li
Fengming Song
author_sort Xi Yuan
title ONAC066, A Stress-Responsive NAC Transcription Activator, Positively Contributes to Rice Immunity Against Magnaprothe oryzae Through Modulating Expression of OsWRKY62 and Three Cytochrome P450 Genes
title_short ONAC066, A Stress-Responsive NAC Transcription Activator, Positively Contributes to Rice Immunity Against Magnaprothe oryzae Through Modulating Expression of OsWRKY62 and Three Cytochrome P450 Genes
title_full ONAC066, A Stress-Responsive NAC Transcription Activator, Positively Contributes to Rice Immunity Against Magnaprothe oryzae Through Modulating Expression of OsWRKY62 and Three Cytochrome P450 Genes
title_fullStr ONAC066, A Stress-Responsive NAC Transcription Activator, Positively Contributes to Rice Immunity Against Magnaprothe oryzae Through Modulating Expression of OsWRKY62 and Three Cytochrome P450 Genes
title_full_unstemmed ONAC066, A Stress-Responsive NAC Transcription Activator, Positively Contributes to Rice Immunity Against Magnaprothe oryzae Through Modulating Expression of OsWRKY62 and Three Cytochrome P450 Genes
title_sort onac066, a stress-responsive nac transcription activator, positively contributes to rice immunity against magnaprothe oryzae through modulating expression of oswrky62 and three cytochrome p450 genes
publisher Frontiers Media S.A.
series Frontiers in Plant Science
issn 1664-462X
publishDate 2021-09-01
description NAC transcriptional factors constitute a large family in rice and some of them have been demonstrated to play crucial roles in rice immunity. The present study investigated the function and mechanism of ONAC066 in rice immunity. ONAC066 shows transcription activator activity that depends on its C-terminal region in rice cells. ONAC066-OE plants exhibited enhanced resistance while ONAC066-Ri and onac066-1 plants showed attenuated resistance to Magnaporthe oryzae. A total of 81 genes were found to be up-regulated in ONAC066-OE plants, and 26 of them were predicted to be induced by M. oryzae. Four OsWRKY genes, including OsWRKY45 and OsWRKY62, were up-regulated in ONAC066-OE plants but down-regulated in ONAC066-Ri plants. ONAC066 bound to NAC core-binding site in OsWRKY62 promoter and activated OsWRKY62 expression, indicating that OsWRKY62 is a ONAC066 target. A set of cytochrome P450 genes were found to be co-expressed with ONAC066 and 5 of them were up-regulated in ONAC066-OE plants but down-regulated in ONAC066-Ri plants. ONAC066 bound to promoters of cytochrome P450 genes LOC_Os02g30110, LOC_Os06g37300, and LOC_Os02g36150 and activated their transcription, indicating that these three cytochrome P450 genes are ONAC066 targets. These results suggest that ONAC066, as a transcription activator, positively contributes to rice immunity through modulating the expression of OsWRKY62 and a set of cytochrome P450 genes to activate defense response.
topic NAC (NAM, ATAF, and CUC)
ONAC066
rice immunity
OsWRKY62
cytochrome P450s
url https://www.frontiersin.org/articles/10.3389/fpls.2021.749186/full
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AT xiyuan onac066astressresponsivenactranscriptionactivatorpositivelycontributestoriceimmunityagainstmagnaprotheoryzaethroughmodulatingexpressionofoswrky62andthreecytochromep450genes
AT huiwang onac066astressresponsivenactranscriptionactivatorpositivelycontributestoriceimmunityagainstmagnaprotheoryzaethroughmodulatingexpressionofoswrky62andthreecytochromep450genes
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AT fengmingsong onac066astressresponsivenactranscriptionactivatorpositivelycontributestoriceimmunityagainstmagnaprotheoryzaethroughmodulatingexpressionofoswrky62andthreecytochromep450genes
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