Functional Studies on the Promoter Region of the Human GTP Cyclohydrolase I Gene

Functional Studies on the Promoter Region of the Human GTP Cyclohydrolase I Gene

We have isolated genomic clones that contained the gene for human GTP cyclohydrolase I. One clone containing the 5'-regulatory region of this gene was further analysed. It encompassed the first exon, parts of the first intron and about 2.6kb of the promoter region. The transcription start site...

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Main Authors: Gütlich Markus, Witter Klaus, Katzenmeier Gerd, Rödl Wolfgang, Wernert Thomas, Ziegler Irmgard, Bacher Adelbert
Format: Article
Language:English
Published: De Gruyter 1995-08-01
Series:Pteridines
Subjects:
keywords: gtp cyclohydrolase i
promoter
5'-race
spl
Online Access:https://doi.org/10.1515/pteridines.1995.6.3.108
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spelling doaj-4cb01306249447c0bfe59b15169597d52021-09-05T13:59:59ZengDe GruyterPteridines0933-48072195-47201995-08-016310811110.1515/pteridines.1995.6.3.108Functional Studies on the Promoter Region of the Human GTP Cyclohydrolase I GeneGütlich Markus0Witter Klaus1Katzenmeier Gerd2Rödl Wolfgang3Wernert Thomas4Ziegler Irmgard5Bacher Adelbert6GSF-Institut für Klinische Molekularbiologie, Marchioninistraße 25, 81377 Müchen, GermanyGSF-Institut für Klinische Molekularbiologie, Marchioninistraße 25, 81377 Müchen, GermanyGSF-Institut für Klinische Molekularbiologie, Marchioninistraße 25, 81377 Müchen, GermanyGSF-Institut für Klinische Molekularbiologie, Marchioninistraße 25, 81377 Müchen, GermanyGSF-Institut für Sägetiergenetik, 85758 Neuherberg, GermanyGSF-Institut für Klinische Molekularbiologie, Marchioninistraße 25, 81377 Müchen, GermanyTechnische Universität München, Lehrstuhl fur Organische Chemie und Biochemie, 85747 Garching, GermanyWe have isolated genomic clones that contained the gene for human GTP cyclohydrolase I. One clone containing the 5'-regulatory region of this gene was further analysed. It encompassed the first exon, parts of the first intron and about 2.6kb of the promoter region. The transcription start site was localised by rapid amplification of cDNA ends (5'-RACE). The 2.6 kb region upstream of the transcription start site showed promoter activity when ligated upstream of a reporter gene. 5'-truncations of the promoter region increased its activity as long as the CAAT and TATA boxes remained unchanged. Several putative Spl responsive elements were located within a GC-rich region close to the transcription start site.https://doi.org/10.1515/pteridines.1995.6.3.108keywords: gtp cyclohydrolase ipromoter5'-racespl
collection DOAJ
language English
format Article
sources DOAJ
author Gütlich Markus
Witter Klaus
Katzenmeier Gerd
Rödl Wolfgang
Wernert Thomas
Ziegler Irmgard
Bacher Adelbert
spellingShingle Gütlich Markus
Witter Klaus
Katzenmeier Gerd
Rödl Wolfgang
Wernert Thomas
Ziegler Irmgard
Bacher Adelbert
Functional Studies on the Promoter Region of the Human GTP Cyclohydrolase I Gene
Pteridines
keywords: gtp cyclohydrolase i
promoter
5'-race
spl
author_facet Gütlich Markus
Witter Klaus
Katzenmeier Gerd
Rödl Wolfgang
Wernert Thomas
Ziegler Irmgard
Bacher Adelbert
author_sort Gütlich Markus
title Functional Studies on the Promoter Region of the Human GTP Cyclohydrolase I Gene
title_short Functional Studies on the Promoter Region of the Human GTP Cyclohydrolase I Gene
title_full Functional Studies on the Promoter Region of the Human GTP Cyclohydrolase I Gene
title_fullStr Functional Studies on the Promoter Region of the Human GTP Cyclohydrolase I Gene
title_full_unstemmed Functional Studies on the Promoter Region of the Human GTP Cyclohydrolase I Gene
title_sort functional studies on the promoter region of the human gtp cyclohydrolase i gene
publisher De Gruyter
series Pteridines
issn 0933-4807
2195-4720
publishDate 1995-08-01
description We have isolated genomic clones that contained the gene for human GTP cyclohydrolase I. One clone containing the 5'-regulatory region of this gene was further analysed. It encompassed the first exon, parts of the first intron and about 2.6kb of the promoter region. The transcription start site was localised by rapid amplification of cDNA ends (5'-RACE). The 2.6 kb region upstream of the transcription start site showed promoter activity when ligated upstream of a reporter gene. 5'-truncations of the promoter region increased its activity as long as the CAAT and TATA boxes remained unchanged. Several putative Spl responsive elements were located within a GC-rich region close to the transcription start site.
topic keywords: gtp cyclohydrolase i
promoter
5'-race
spl
url https://doi.org/10.1515/pteridines.1995.6.3.108
work_keys_str_mv AT gutlichmarkus functionalstudiesonthepromoterregionofthehumangtpcyclohydrolaseigene
AT witterklaus functionalstudiesonthepromoterregionofthehumangtpcyclohydrolaseigene
AT katzenmeiergerd functionalstudiesonthepromoterregionofthehumangtpcyclohydrolaseigene
AT rodlwolfgang functionalstudiesonthepromoterregionofthehumangtpcyclohydrolaseigene
AT wernertthomas functionalstudiesonthepromoterregionofthehumangtpcyclohydrolaseigene
AT zieglerirmgard functionalstudiesonthepromoterregionofthehumangtpcyclohydrolaseigene
AT bacheradelbert functionalstudiesonthepromoterregionofthehumangtpcyclohydrolaseigene
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