A redox-sensitive luciferase assay for determining the localization and topology of endoplasmic reticulum proteins.

A redox-sensitive luciferase assay for determining the localization and topology of endoplasmic reticulum proteins.

Correct localization and transmembrane topology are crucial for the proteins residing and functioning in the endoplasmic reticulum (ER). We have developed a rapid and convenient assay, based on the redox-sensitive luciferase from Gaussia princeps (Gluc) and green fluorescence protein (GFP), to deter...

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Main Authors: Hai-Yin Li, Xue-Ming Zheng, Mei-Xia Che, Hong-Yu Hu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3329452?pdf=render
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spelling doaj-4caa4fa1fd48457c83bdb997764975c42020-11-24T21:45:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3562810.1371/journal.pone.0035628A redox-sensitive luciferase assay for determining the localization and topology of endoplasmic reticulum proteins.Hai-Yin LiXue-Ming ZhengMei-Xia CheHong-Yu HuCorrect localization and transmembrane topology are crucial for the proteins residing and functioning in the endoplasmic reticulum (ER). We have developed a rapid and convenient assay, based on the redox-sensitive luciferase from Gaussia princeps (Gluc) and green fluorescence protein (GFP), to determine the localization or topology of ER proteins. Using the tandem Gluc-GFP reporter fused to different positions of a target protein, we successfully characterized the topologies of two ER transmembrane proteins Herp and HRD1 that are involved in the ER quality control system. This assay method may also be applicable to the proteins in secretory pathway, plasma membrane, and other compartments of cells.http://europepmc.org/articles/PMC3329452?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Hai-Yin Li
Xue-Ming Zheng
Mei-Xia Che
Hong-Yu Hu
spellingShingle Hai-Yin Li
Xue-Ming Zheng
Mei-Xia Che
Hong-Yu Hu
A redox-sensitive luciferase assay for determining the localization and topology of endoplasmic reticulum proteins.
PLoS ONE
author_facet Hai-Yin Li
Xue-Ming Zheng
Mei-Xia Che
Hong-Yu Hu
author_sort Hai-Yin Li
title A redox-sensitive luciferase assay for determining the localization and topology of endoplasmic reticulum proteins.
title_short A redox-sensitive luciferase assay for determining the localization and topology of endoplasmic reticulum proteins.
title_full A redox-sensitive luciferase assay for determining the localization and topology of endoplasmic reticulum proteins.
title_fullStr A redox-sensitive luciferase assay for determining the localization and topology of endoplasmic reticulum proteins.
title_full_unstemmed A redox-sensitive luciferase assay for determining the localization and topology of endoplasmic reticulum proteins.
title_sort redox-sensitive luciferase assay for determining the localization and topology of endoplasmic reticulum proteins.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2012-01-01
description Correct localization and transmembrane topology are crucial for the proteins residing and functioning in the endoplasmic reticulum (ER). We have developed a rapid and convenient assay, based on the redox-sensitive luciferase from Gaussia princeps (Gluc) and green fluorescence protein (GFP), to determine the localization or topology of ER proteins. Using the tandem Gluc-GFP reporter fused to different positions of a target protein, we successfully characterized the topologies of two ER transmembrane proteins Herp and HRD1 that are involved in the ER quality control system. This assay method may also be applicable to the proteins in secretory pathway, plasma membrane, and other compartments of cells.
url http://europepmc.org/articles/PMC3329452?pdf=render
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