Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway
Abstract Objective We aimed to investigate the therapeutic effects of Moringa oleifera leaf extracts on osteogenic induction of rat bone marrow mesenchymal stem cells (BMSCs) following peroxidative damage and to explore the underlying mechanisms. Methods Conditioned medium was used to induce osteoge...
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doaj-4c70d1ca4fe44b0ba4cf8ccca9868c3d2021-02-21T12:26:54ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2021-02-0116111410.1186/s13018-021-02284-xMoringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathwayMeiling Liu0Haifeng Ding1Hongzhi Wang2Manfeng Wang3Xiaowei Wu4Lu Gan5Luyang Cheng6Xianglu Li7Department of Geriatrics, Second Affiliated Hospital of Harbin Medical UniversityDepartment of Geriatrics, Second Affiliated Hospital of Harbin Medical UniversityDepartment of Geriatrics, Second Affiliated Hospital of Harbin Medical UniversityDepartment of Geriatrics, Second Affiliated Hospital of Harbin Medical UniversityDepartment of Geriatrics, Second Affiliated Hospital of Harbin Medical UniversityDepartment of Geriatrics, Second Affiliated Hospital of Harbin Medical UniversityDepartment of Geriatrics, Second Affiliated Hospital of Harbin Medical UniversityDepartment of Geriatrics, Second Affiliated Hospital of Harbin Medical UniversityAbstract Objective We aimed to investigate the therapeutic effects of Moringa oleifera leaf extracts on osteogenic induction of rat bone marrow mesenchymal stem cells (BMSCs) following peroxidative damage and to explore the underlying mechanisms. Methods Conditioned medium was used to induce osteogenic differentiation of BMSCs, which were treated with H2O2, Moringa oleifera leaf extracts-containing serum, or the phosphatidyl inositol-3 kinase (PI3K) inhibitor wortmannin, alone or in combination. Cell viability was measured using the MTT assay. Cell cycle was assayed using flow cytometry. Expression levels of Akt, phosphorylated (p)Akt, Foxo1, and cleaved caspase-3 were analyzed using western blot analysis. The mRNA levels of osteogenesis-associated genes, including alkaline phosphatase (ALP), collagen І, osteopontin (OPN), and Runx2, were detected using qRT-PCR. Reactive oxygen species (ROS) and malondialdehyde (MDA) levels, as well as superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and ALP activity were detected using commercially available kits. Osteogenic differentiation capability was determined using alizarin red staining. Results During osteogenic induction of rat BMSCs, H2O2 reduced cell viability and proliferation, inhibited osteogenesis, increased ROS and MDA levels, and decreased SOD and GSH-PX activity. H2O2 significantly reduced pAkt and Foxo1 expression, and increased cleaved caspase-3 levels in BMSCs. Additional treatments with Moringa oleifera leaf extracts partially reversed the H2O2-induced changes. Wortmannin partially attenuated the effects of Moringa oleifera leaf extracts on protein expression of Foxo1, pAkt, and cleaved caspase-3, as well as mRNA levels of osteogenesis-associated genes. Conclusion Moringa oleifera leaf extracts ameliorate peroxidative damage and enhance osteogenic induction of rat BMSCs by activating the PI3K/Akt/Foxo1 pathway.https://doi.org/10.1186/s13018-021-02284-xMoringa oleifera leaf extractsBone marrow mesenchymal stem cellsOsteogenic inductionHydrogen peroxidePI3K/Akt pathwayFoxo1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Meiling Liu Haifeng Ding Hongzhi Wang Manfeng Wang Xiaowei Wu Lu Gan Luyang Cheng Xianglu Li |
spellingShingle |
Meiling Liu Haifeng Ding Hongzhi Wang Manfeng Wang Xiaowei Wu Lu Gan Luyang Cheng Xianglu Li Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway Journal of Orthopaedic Surgery and Research Moringa oleifera leaf extracts Bone marrow mesenchymal stem cells Osteogenic induction Hydrogen peroxide PI3K/Akt pathway Foxo1 |
author_facet |
Meiling Liu Haifeng Ding Hongzhi Wang Manfeng Wang Xiaowei Wu Lu Gan Luyang Cheng Xianglu Li |
author_sort |
Meiling Liu |
title |
Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway |
title_short |
Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway |
title_full |
Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway |
title_fullStr |
Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway |
title_full_unstemmed |
Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway |
title_sort |
moringa oleifera leaf extracts protect bmsc osteogenic induction following peroxidative damage by activating the pi3k/akt/foxo1 pathway |
publisher |
BMC |
series |
Journal of Orthopaedic Surgery and Research |
issn |
1749-799X |
publishDate |
2021-02-01 |
description |
Abstract Objective We aimed to investigate the therapeutic effects of Moringa oleifera leaf extracts on osteogenic induction of rat bone marrow mesenchymal stem cells (BMSCs) following peroxidative damage and to explore the underlying mechanisms. Methods Conditioned medium was used to induce osteogenic differentiation of BMSCs, which were treated with H2O2, Moringa oleifera leaf extracts-containing serum, or the phosphatidyl inositol-3 kinase (PI3K) inhibitor wortmannin, alone or in combination. Cell viability was measured using the MTT assay. Cell cycle was assayed using flow cytometry. Expression levels of Akt, phosphorylated (p)Akt, Foxo1, and cleaved caspase-3 were analyzed using western blot analysis. The mRNA levels of osteogenesis-associated genes, including alkaline phosphatase (ALP), collagen І, osteopontin (OPN), and Runx2, were detected using qRT-PCR. Reactive oxygen species (ROS) and malondialdehyde (MDA) levels, as well as superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and ALP activity were detected using commercially available kits. Osteogenic differentiation capability was determined using alizarin red staining. Results During osteogenic induction of rat BMSCs, H2O2 reduced cell viability and proliferation, inhibited osteogenesis, increased ROS and MDA levels, and decreased SOD and GSH-PX activity. H2O2 significantly reduced pAkt and Foxo1 expression, and increased cleaved caspase-3 levels in BMSCs. Additional treatments with Moringa oleifera leaf extracts partially reversed the H2O2-induced changes. Wortmannin partially attenuated the effects of Moringa oleifera leaf extracts on protein expression of Foxo1, pAkt, and cleaved caspase-3, as well as mRNA levels of osteogenesis-associated genes. Conclusion Moringa oleifera leaf extracts ameliorate peroxidative damage and enhance osteogenic induction of rat BMSCs by activating the PI3K/Akt/Foxo1 pathway. |
topic |
Moringa oleifera leaf extracts Bone marrow mesenchymal stem cells Osteogenic induction Hydrogen peroxide PI3K/Akt pathway Foxo1 |
url |
https://doi.org/10.1186/s13018-021-02284-x |
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