Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway

Abstract Objective We aimed to investigate the therapeutic effects of Moringa oleifera leaf extracts on osteogenic induction of rat bone marrow mesenchymal stem cells (BMSCs) following peroxidative damage and to explore the underlying mechanisms. Methods Conditioned medium was used to induce osteoge...

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Main Authors: Meiling Liu, Haifeng Ding, Hongzhi Wang, Manfeng Wang, Xiaowei Wu, Lu Gan, Luyang Cheng, Xianglu Li
Format: Article
Language:English
Published: BMC 2021-02-01
Series:Journal of Orthopaedic Surgery and Research
Subjects:
Online Access:https://doi.org/10.1186/s13018-021-02284-x
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spelling doaj-4c70d1ca4fe44b0ba4cf8ccca9868c3d2021-02-21T12:26:54ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2021-02-0116111410.1186/s13018-021-02284-xMoringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathwayMeiling Liu0Haifeng Ding1Hongzhi Wang2Manfeng Wang3Xiaowei Wu4Lu Gan5Luyang Cheng6Xianglu Li7Department of Geriatrics, Second Affiliated Hospital of Harbin Medical UniversityDepartment of Geriatrics, Second Affiliated Hospital of Harbin Medical UniversityDepartment of Geriatrics, Second Affiliated Hospital of Harbin Medical UniversityDepartment of Geriatrics, Second Affiliated Hospital of Harbin Medical UniversityDepartment of Geriatrics, Second Affiliated Hospital of Harbin Medical UniversityDepartment of Geriatrics, Second Affiliated Hospital of Harbin Medical UniversityDepartment of Geriatrics, Second Affiliated Hospital of Harbin Medical UniversityDepartment of Geriatrics, Second Affiliated Hospital of Harbin Medical UniversityAbstract Objective We aimed to investigate the therapeutic effects of Moringa oleifera leaf extracts on osteogenic induction of rat bone marrow mesenchymal stem cells (BMSCs) following peroxidative damage and to explore the underlying mechanisms. Methods Conditioned medium was used to induce osteogenic differentiation of BMSCs, which were treated with H2O2, Moringa oleifera leaf extracts-containing serum, or the phosphatidyl inositol-3 kinase (PI3K) inhibitor wortmannin, alone or in combination. Cell viability was measured using the MTT assay. Cell cycle was assayed using flow cytometry. Expression levels of Akt, phosphorylated (p)Akt, Foxo1, and cleaved caspase-3 were analyzed using western blot analysis. The mRNA levels of osteogenesis-associated genes, including alkaline phosphatase (ALP), collagen І, osteopontin (OPN), and Runx2, were detected using qRT-PCR. Reactive oxygen species (ROS) and malondialdehyde (MDA) levels, as well as superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and ALP activity were detected using commercially available kits. Osteogenic differentiation capability was determined using alizarin red staining. Results During osteogenic induction of rat BMSCs, H2O2 reduced cell viability and proliferation, inhibited osteogenesis, increased ROS and MDA levels, and decreased SOD and GSH-PX activity. H2O2 significantly reduced pAkt and Foxo1 expression, and increased cleaved caspase-3 levels in BMSCs. Additional treatments with Moringa oleifera leaf extracts partially reversed the H2O2-induced changes. Wortmannin partially attenuated the effects of Moringa oleifera leaf extracts on protein expression of Foxo1, pAkt, and cleaved caspase-3, as well as mRNA levels of osteogenesis-associated genes. Conclusion Moringa oleifera leaf extracts ameliorate peroxidative damage and enhance osteogenic induction of rat BMSCs by activating the PI3K/Akt/Foxo1 pathway.https://doi.org/10.1186/s13018-021-02284-xMoringa oleifera leaf extractsBone marrow mesenchymal stem cellsOsteogenic inductionHydrogen peroxidePI3K/Akt pathwayFoxo1
collection DOAJ
language English
format Article
sources DOAJ
author Meiling Liu
Haifeng Ding
Hongzhi Wang
Manfeng Wang
Xiaowei Wu
Lu Gan
Luyang Cheng
Xianglu Li
spellingShingle Meiling Liu
Haifeng Ding
Hongzhi Wang
Manfeng Wang
Xiaowei Wu
Lu Gan
Luyang Cheng
Xianglu Li
Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway
Journal of Orthopaedic Surgery and Research
Moringa oleifera leaf extracts
Bone marrow mesenchymal stem cells
Osteogenic induction
Hydrogen peroxide
PI3K/Akt pathway
Foxo1
author_facet Meiling Liu
Haifeng Ding
Hongzhi Wang
Manfeng Wang
Xiaowei Wu
Lu Gan
Luyang Cheng
Xianglu Li
author_sort Meiling Liu
title Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway
title_short Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway
title_full Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway
title_fullStr Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway
title_full_unstemmed Moringa oleifera leaf extracts protect BMSC osteogenic induction following peroxidative damage by activating the PI3K/Akt/Foxo1 pathway
title_sort moringa oleifera leaf extracts protect bmsc osteogenic induction following peroxidative damage by activating the pi3k/akt/foxo1 pathway
publisher BMC
series Journal of Orthopaedic Surgery and Research
issn 1749-799X
publishDate 2021-02-01
description Abstract Objective We aimed to investigate the therapeutic effects of Moringa oleifera leaf extracts on osteogenic induction of rat bone marrow mesenchymal stem cells (BMSCs) following peroxidative damage and to explore the underlying mechanisms. Methods Conditioned medium was used to induce osteogenic differentiation of BMSCs, which were treated with H2O2, Moringa oleifera leaf extracts-containing serum, or the phosphatidyl inositol-3 kinase (PI3K) inhibitor wortmannin, alone or in combination. Cell viability was measured using the MTT assay. Cell cycle was assayed using flow cytometry. Expression levels of Akt, phosphorylated (p)Akt, Foxo1, and cleaved caspase-3 were analyzed using western blot analysis. The mRNA levels of osteogenesis-associated genes, including alkaline phosphatase (ALP), collagen І, osteopontin (OPN), and Runx2, were detected using qRT-PCR. Reactive oxygen species (ROS) and malondialdehyde (MDA) levels, as well as superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and ALP activity were detected using commercially available kits. Osteogenic differentiation capability was determined using alizarin red staining. Results During osteogenic induction of rat BMSCs, H2O2 reduced cell viability and proliferation, inhibited osteogenesis, increased ROS and MDA levels, and decreased SOD and GSH-PX activity. H2O2 significantly reduced pAkt and Foxo1 expression, and increased cleaved caspase-3 levels in BMSCs. Additional treatments with Moringa oleifera leaf extracts partially reversed the H2O2-induced changes. Wortmannin partially attenuated the effects of Moringa oleifera leaf extracts on protein expression of Foxo1, pAkt, and cleaved caspase-3, as well as mRNA levels of osteogenesis-associated genes. Conclusion Moringa oleifera leaf extracts ameliorate peroxidative damage and enhance osteogenic induction of rat BMSCs by activating the PI3K/Akt/Foxo1 pathway.
topic Moringa oleifera leaf extracts
Bone marrow mesenchymal stem cells
Osteogenic induction
Hydrogen peroxide
PI3K/Akt pathway
Foxo1
url https://doi.org/10.1186/s13018-021-02284-x
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